Slusarczyk J, Hansson B G, Nordenfelt E, Krawczyński K, Karwowska S, Knap J
J Med Virol. 1985 Feb;15(2):105-12. doi: 10.1002/jmv.1890150202.
The excretion of hepatitis A virus (HAV) in stools from 30 patients with clinically overt hepatitis A infection on the day of their admission to the hospital was determined and compared with the dynamics and values of biochemical indices of hepatocyte injury as well as with the immune response to HAV. Virus was found in 16 out of 30 stools (53%) collected within 1 week after the appearance of clinical symptoms. In sera obtained on the day of hospitalization both IgM and IgA anti-HAV were detected in all of the 30 patients, while IgG anti-HAV were found in 20 (67%). There was a correlation between HAV excretion and increasing SGPT upon admission to hospital, while the level of SGPT or bilirubin as well as presence or absence of IgG anti-HAV did not correlate with excretion of HAV. HAV from stools was characterized morphologically and physicochemically. The majority of particles visualized by immune electron microscopy had electrondense appearance, while electron-lucid particles were only occasionally encountered. Isopycnic banding of HAV in CsCl revealed a broad range of densities with HAV activity. Rebanding of pooled fractions containing HAV revealed peak amounts of the virus in fractions with densities 1.32-1.33 gm/cm3.
对30例临床显性甲型肝炎感染患者入院当天粪便中甲型肝炎病毒（HAV）的排泄情况进行了测定，并与肝细胞损伤的生化指标动态变化及数值，以及对HAV的免疫反应进行了比较。在临床症状出现后1周内采集的30份粪便样本中，有16份（53%）检测到病毒。住院当天采集的血清中，所有30例患者均检测到IgM和IgA抗-HAV，而20例（67%）检测到IgG抗-HAV。入院时HAV排泄与谷丙转氨酶（SGPT）升高之间存在相关性，而SGPT或胆红素水平以及IgG抗-HAV的有无与HAV排泄无关。对粪便中的HAV进行了形态学和理化特性分析。免疫电子显微镜观察到的大多数颗粒呈电子致密外观，而电子透明颗粒仅偶尔出现。在氯化铯中对HAV进行等密度梯度离心显示，具有HAV活性的密度范围很广。对含有HAV的合并组分进行再分级显示，密度为1.32 - 1.33 g/cm³的组分中病毒含量最高。
