通过改良的免疫球蛋白M抗甲型肝炎病毒竞争性结合放射免疫测定法进行甲型病毒性肝炎的血清学诊断。
Serodiagnosis of viral hepatitis A by a modified competitive binding radioimmunoassay for immunoglobulin M anti-hepatitis A virus.
作者信息
Bradley D W, Fields H A, McCaustland K A, Maynard J E, Decker R H, Whittington R, Overby L R
出版信息
J Clin Microbiol. 1979 Jan;9(1):120-7. doi: 10.1128/jcm.9.1.120-127.1979.
Abstract
A competitive binding radioimmunoassay (CBA) for antibody to hepatitis A virus (HAV) was evaluated and compared with a standard solid-phase radioimmunoassay for anti-HAV, CBA was found to be sensitive and specific for the detection of anti-HAV, as demonstrated by the 98% concordance of CBA and solid-phase radioimmunoassay test results. The standard CBA test was modified for the differential detection of acute (immunoglobulin M) and convalescent (immunoglobulin G) anti-HAV by incorporation of a step in which immunoglobulin G anti-HAV was preferentially absorbed with S. aureus cells (protein A). The modified CBA test was shown to be capable of differentiating between acute- and convalescent-phase sera. The modified CBAM test was able to detect immunoglobulin M anti-HAV up to approximately 4 weeks after the onset of illness.
摘要
对甲型肝炎病毒（HAV）抗体的竞争性结合放射免疫测定（CBA）进行了评估，并与抗-HAV的标准固相放射免疫测定进行了比较。发现CBA在检测抗-HAV方面具有敏感性和特异性，CBA与固相放射免疫测定测试结果的一致性为98%，证明了这一点。通过加入一步，其中抗-HAV免疫球蛋白G被金黄色葡萄球菌细胞（蛋白A）优先吸收，对标准CBA试验进行了改进，用于急性（免疫球蛋白M）和恢复期（免疫球蛋白G）抗-HAV的鉴别检测。改进后的CBA试验能够区分急性期和恢复期血清。改进后的CBAM试验能够在发病后约4周内检测到抗-HAV免疫球蛋白M。
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