黑猩猩肝脏、胆汁和粪便中的甲型肝炎抗原颗粒。

Hepatitis A antigen particles in liver, bile, and stool of chimpanzees.

作者信息

Schulman A N, Dienstag J L, Jackson D R, Hoofnagle J H, Gerety R J, Purcell R H, Barker L F

出版信息

J Infect Dis. 1976 Jul;134(1):80-4. doi: 10.1093/infdis/134.1.80.

DOI:10.1093/infdis/134.1.80

Abstract

Virus-like hepatitis A antigen (HA Ag) particles, presumably hepatitis A virus, were isolated from the liver, bile, and stool of three chimpanzees that had been infected with stool filtrates containing HA Ag particles. Specimens of serum, stool, liver biopsy material, and bile were obtained at selected intervals during the experiment. The animals developed mild hepatitis 19-21 days after inoculation, and antibody to HA Ag appeared de novo in their convalescent-phase serum. During acute illness, virus-like particles similar to the HA Ag particle were seen in liver cell cytoplasm by electron microscopy. HA Ag particles were detected by immune electron microscopy and a new radioimmunoassay in isopycnically banded samples of liver, bile, and stool. HA Ag particles were found at densities of 1.29-1.39 g/cm3, but the major peak density for antigen particles in samples of liver, bile, and stool was approximately 1.34 g/cm3. The fact that HA Ag particles can be recovered from chimpanzee liver, bile, and stool makes these potentially important sources of infectious and antigenic materials.

摘要

从三只感染了含有甲型肝炎抗原（HA Ag）颗粒的粪便滤液的黑猩猩的肝脏、胆汁和粪便中分离出了病毒样甲型肝炎抗原（HA Ag）颗粒，推测为甲型肝炎病毒。在实验过程中的选定时间间隔采集血清、粪便、肝活检材料和胆汁样本。动物在接种后19 - 21天出现轻度肝炎，并且在恢复期血清中重新出现了针对HA Ag的抗体。在急性疾病期间，通过电子显微镜在肝细胞胞质中观察到与HA Ag颗粒相似的病毒样颗粒。通过免疫电子显微镜和一种新的放射免疫分析法在等密度离心分离的肝脏、胆汁和粪便样本中检测到了HA Ag颗粒。HA Ag颗粒的密度为1.29 - 1.39 g/cm³，但肝脏、胆汁和粪便样本中抗原颗粒的主要峰值密度约为1.34 g/cm³。HA Ag颗粒能够从黑猩猩的肝脏、胆汁和粪便中回收这一事实使其成为潜在的重要感染性和抗原性物质来源。