Esposito J J, Obijeski J F, Nakano J H
J Med Virol. 1977;1(2):95-110. doi: 10.1002/jmv.1890010203.
The structural proteins in purified preparations of variola, monkeypox, and vaccinia viruses were separated and compared by using a high resolution SDS-polyacrylamide gel electrophoresis system. About 30 proteins were resolved for each virus by autoradiography of longitudinally-sliced gel rods. Although the autoradioelectropherograms of each virus were similar, it was possible to differentiate them by their unique protein pattern in the 30,000 to 40,000 molecular weight region of the gels. A single virion glycoprotein (mol. wt. = 38 X 10(3)) and a virion phosphoprotein (mol. wt. = 12 X 10(3)) were associated with each of the virus preparations. Cross-absorbed monospecific immune sera against variola, monkeypox, and vaccinia virus-infected cells were used in immunodiffusion tests to precipitate radiolabeled, homologous, soluble antigen proteins. The predominant antigen protein associated with each immunospecific precipitate had a molecular weight of approximately 73,000.
利用高分辨率SDS-聚丙烯酰胺凝胶电泳系统，对纯化的天花病毒、猴痘病毒和痘苗病毒制剂中的结构蛋白进行了分离和比较。通过对纵向切片的凝胶棒进行放射自显影，每种病毒大约分辨出30种蛋白质。虽然每种病毒的放射电泳图谱相似，但通过凝胶中分子量在30000至40000区域内独特的蛋白质模式，可以区分它们。每种病毒制剂都与一种单一的病毒粒子糖蛋白（分子量=38×10³）和一种病毒粒子磷蛋白（分子量=12×10³）相关。针对天花、猴痘和痘苗病毒感染细胞的交叉吸收单特异性免疫血清用于免疫扩散试验，以沉淀放射性标记的同源可溶性抗原蛋白。与每种免疫特异性沉淀物相关的主要抗原蛋白分子量约为73000。
