通过放射免疫测定法对天花病毒和猴痘病毒连续抗血清特异性的研究。
A study of the specificities of sequential antisera to variola and monkeypox viruses by radioimmunoassay.
作者信息
Walls H H, Ziegler D W, Nakano J H
出版信息
Bull World Health Organ. 1980;58(1):131-8.
The specificities of antisera during development of the humoral antibody response to poxvirus antigens were examined in monkeys injected with chimp-9 whitepox virus or monkeypox virus. Sera were obtained from 3 African green (vervet) monkeys inoculated with chimp-9 whitepox virus, 1 rhesus monkey inoculated with monkeypox virus, and 2 rhesus monkeys inoculated with soluble monkeypox viral antigen. The sequentially obtained sera from each animal were adsorbed with uninfected chicken chorioallantoic membranes (CAM) or vaccinia virus-infected CAM. The adsorbed sera were tested by radioimmunoassay to determine the specificity of the residual antibodies to vaccinia, variola, and monkeypox viruses. The adsorbed sera at different stages of the immune response showed increasing specificity with time after inoculation. Generally, antibodies in sera collected earlier than 21-27 days after immunization could not be identified after adsorption, but late sera could be identified unequivocally.
在注射了黑猩猩9型白痘病毒或猴痘病毒的猴子中，研究了体液抗体应答针对痘病毒抗原产生过程中抗血清的特异性。从3只接种了黑猩猩9型白痘病毒的非洲绿猴（绿猴）、1只接种了猴痘病毒的恒河猴以及2只接种了可溶性猴痘病毒抗原的恒河猴身上采集血清。从每只动物身上按顺序采集的血清，用未感染的鸡胚绒毛尿囊膜（CAM）或痘苗病毒感染的CAM进行吸附处理。通过放射免疫测定法检测吸附后的血清，以确定残留抗体针对痘苗病毒、天花病毒和猴痘病毒的特异性。免疫应答不同阶段的吸附后血清，在接种后随时间推移显示出特异性增加。一般来说，免疫后21 - 27天之前采集的血清中的抗体，吸附后无法鉴定，但后期血清可以明确鉴定。
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