Department of Plant Pathology and Microbiology, Texas A&M University, 2132 TAMU, College Station, TX 77843, USA.
Mol Plant Pathol. 2005 Sep 1;6(5):491-502. doi: 10.1111/j.1364-3703.2005.00301.x.
SUMMARY Taxonomy: Tomato bushy stunt virus (TBSV) (Fig. 1) is the type species of the plant-infecting Tombusvirus genus in the family Tombusviridae. Physical properties: TBSV virions are non-enveloped icosahedral T = 3 particles assembled from 180 coat protein subunits (42 kDa) whose arrangement causes a granular appearance on the surface structure. The particles are approximately 33 nm in diameter and composed of 17% ribonucleic acid and 83% protein. Encapsidated within the virion is the TBSV genome that consists of a positive-sense single-stranded RNA of approximately 4.8 kb, which lacks the 5'-cap or 3'-poly(A) tail typical for eukaryotic mRNAs.
In nature, TBSV has a fairly restricted host range, mostly encompassing a few dicotyledonous species in separate families, and affected agricultural crops comprise primarily vegetables. The experimental host range is broad, with over 120 plant species in more than 20 different families reported to be susceptible although in most plants the infection often remains localized around the site of entry. The differences between hosts with regards to requirements for cell-to-cell and long-distance movement have led to the development of TBSV as an attractive model system to obtain general insights into RNA transport through plants.
SYMPTOMS induced by TBSV are largely dependent on the host genotype; they can vary from necrotic and chlorotic lesions, to a systemic mild or severe mosaic, or they may culminate in a lethal necrosis. The original TBSV isolates from tomato plants caused a mottle, crinkle and downward curling of leaves with the youngest leaves exhibiting tip necrosis upon systemic infection. Tomato fruit yield can be greatly reduced by virus infection. Plants may be stunted and a proliferation of lateral shoots leads to a bushy appearance of the infected tomato plants, hence the nomenclature of the pathogen. Useful sites: http://image.fs.uidaho.edu/vide/descr825.htm; http://www.ictvdb.rothamsted.ac.uk/ICTVdB/74010001.htm (general information); http://mmtsb.scripps.edu/viper/info_page.php?vipPDB=2tbv (structural information).
概述 分类：番茄丛矮病毒（TBSV）（图 1）是呼肠孤病毒科呼肠孤病毒属中侵染植物的病毒的模式种。 物理特性：TBSV 病毒粒子是非包膜的二十面体 T = 3 粒子，由 180 个外壳蛋白亚基（42 kDa）组装而成，其排列导致表面结构呈颗粒状。粒子直径约为 33nm，由 17%的核糖核酸和 83%的蛋白质组成。包裹在病毒粒子内的是 TBSV 基因组，它由一条约 4.8kb 的正链单链 RNA 组成，该 RNA 缺乏真核 mRNA 典型的 5'-帽或 3'-poly(A)尾。 宿主范围：在自然界中，TBSV 的宿主范围相当有限，主要包括几个不同科的双子叶植物，受感染的农作物主要是蔬菜。实验宿主范围很广，据报道，超过 120 种植物来自 20 多个不同的科，对其敏感，但在大多数植物中，感染通常局限于进入部位周围。宿主在细胞间和长距离运动方面的需求差异导致 TBSV 成为一个有吸引力的模型系统，用于获得对 RNA 通过植物运输的一般见解。 症状：TBSV 引起的症状在很大程度上取决于宿主基因型；它们可以从坏死和黄化病变，到全身性轻度或严重的镶嵌，或者最终导致致死性坏死。最初从番茄植物中分离出的 TBSV 会导致叶片出现斑驳、皱缩和向下卷曲，系统感染时最年轻的叶片会出现尖端坏死。病毒感染会大大降低番茄果实的产量。植株可能会矮小，侧芽大量增殖会导致感染的番茄植株出现丛生状，因此病原体有这个名称。 有用的网站：http://image.fs.uidaho.edu/vide/descr825.htm；http://www.ictvdb.rothamsted.ac.uk/ICTVdB/74010001.htm（一般信息）；http://mmtsb.scripps.edu/viper/info_page.php?vipPDB=2tbv（结构信息）。
