Chan A W, Leong F W, Langan M C, Schanley D L, Penetrante M L
Research Institute on Alcoholism, New York State Division of Alcoholism and Alcohol Abuse, Buffalo, NY 14203.
Pharmacol Biochem Behav. 1991 Feb;38(2):433-9. doi: 10.1016/0091-3057(91)90303-j.
Two criteria need to be satisfied in the demonstration of cross-dependence to chlordiazepoxide (CDP) in ethanol-dependent mice. These are the ability of CDP to suppress ethanol withdrawal and to maintain the dependent state. In this study, mice which had been fed chronically an ethanol diet followed by two days of CDP diet treatment had more severe CDP withdrawal signs induced by Ro15-1788 than drug-naive mice which were similarly exposed to the CDP diet treatment. The data indicate that CDP can maintain the dependent state acquired from the prior ethanol treatment. Alternatively, the prior ethanol treatment would have facilitated the development of CDP dependence, but it was not deemed likely. Three behavioral tests, namely, runway, sleep time, and drug-induced hypothermia, were used to test whether CDP could maintain the ethanol tolerance acquired from the prior ethanol treatment. The runway test showed that CDP could maintain the previously acquired ethanol tolerance. However, interpretations of the data from the sleep time and hypothermia tests are more equivocal because of factors such as peak tolerance, differential rates of development and dissipation of ethanol (or CDP) tolerance as determined by different behavioral tests.
在证明乙醇依赖小鼠对氯氮卓（CDP）存在交叉依赖性时，需要满足两个标准。这两个标准是CDP抑制乙醇戒断和维持依赖状态的能力。在本研究中，长期喂食乙醇饮食后再进行两天CDP饮食处理的小鼠，与同样接受CDP饮食处理的未接触过药物的小鼠相比，被Ro15 - 1788诱导出更严重的CDP戒断症状。数据表明，CDP可以维持从先前乙醇处理中获得的依赖状态。或者，先前的乙醇处理可能促进了CDP依赖性的发展，但这种可能性不大。使用了三种行为测试，即跑道试验、睡眠时间和药物诱导的体温过低，来测试CDP是否能维持从先前乙醇处理中获得的乙醇耐受性。跑道试验表明，CDP可以维持先前获得的乙醇耐受性。然而，由于诸如峰值耐受性、不同行为测试所确定的乙醇（或CDP）耐受性发展和消散的差异速率等因素，对睡眠时间和体温过低测试数据的解释更具不确定性。
