Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Jpn J Ophthalmol. 2010 May;54(3):215-20. doi: 10.1007/s10384-009-0786-0. Epub 2010 Jun 25.
To use synthesized onion lachrymatory factor (SOLF) to investigate age-related changes in reflex-tear secretion and ocular-surface sensation.
We separated 91 healthy volunteers into four groups: groups A, age 20-29 years; B, 30-39; C, 40-49; and D, older than 50 years. We exposed one eye of each subject to SOLF and measured the elapsed time until the subject's limit of irritation tolerance (TLI) was reached and an increase in the tear meniscus radius (DeltaR). After the SOLF stimulus, corneal sensitivity was examined by Cochet-Bonnet esthesiometry (CB), and reflex-tear secretion was examined by the Schirmer I-test (ST).
TLI was significantly shorter in group A than in the other groups (P < 0.0001), and the groups B and D also differed significantly from each other (P = 0.0013). The increase in DeltaR was significantly greater in group A than in group C (P = 0.0306) or D (P < 0.0001), and groups B (P = 0.0002) and C (P = 0.0308) also differed significantly from group D. There were no significant intergroup differences in the CB and ST results.
An age-related decrease in reflex-tear secretion and ocular-surface sensation was revealed by the SOLF test but could not be detected by either CB or the ST.
使用合成洋葱刺激性因子（SOLF）研究与年龄相关的反射性泪液分泌和眼表面感觉的变化。
我们将 91 名健康志愿者分为四组：A 组，年龄 20-29 岁；B 组，30-39 岁；C 组，40-49 岁；D 组，年龄大于 50 岁。我们将每位受试者的一只眼睛暴露于 SOLF，并测量直到受试者达到刺激耐受极限（TLI）和泪膜新月半径（DeltaR）增加的时间。在 SOLF 刺激后，使用 Cochet-Bonnet 触诊仪（CB）检查角膜敏感性，并用 Schirmer I 试验（ST）检查反射性泪液分泌。
A 组的 TLI 明显短于其他组（P < 0.0001），B 组和 D 组之间也有显著差异（P = 0.0013）。A 组的 DeltaR 增加明显大于 C 组（P = 0.0306）或 D 组（P < 0.0001），B 组（P = 0.0002）和 C 组（P = 0.0308）也与 D 组有显著差异。CB 和 ST 结果在组间无显著差异。
SOLF 测试显示与年龄相关的反射性泪液分泌和眼表面感觉下降，但 CB 或 ST 无法检测到。
