分批发酵和固态发酵生产脂肪酶和生物表面活性剂。
Simultaneous production of lipases and biosurfactants by submerged and solid-state bioprocesses.
机构信息
Laboratory of Fermentations, Course of Food Engineering, College of Engineering and Architecture, University of Passo Fundo, Campus I, km 171, BR 285, P.O. Box 611, CEP 99001-970, Passo Fundo, RS, Brazil.
出版信息
Bioresour Technol. 2010 Nov;101(21):8308-14. doi: 10.1016/j.biortech.2010.05.086. Epub 2010 Jul 1.
Lipases and biosurfactants are compounds produced by microorganisms generally involved in the metabolization of oil substrates. However, the relationship between the production of lipases and biosurfactants has not been established yet. Therefore, this study aimed to evaluate the correlation between production of lipases and biosurfactants by submerged (SmgB) and solid-state bioprocess (SSB) using Aspergillus spp., which were isolated from a soil contaminated by diesel oil. SSB had the highest production of lipases, with lipolytic activities of 25.22U, while SmgB had 4.52U. The production of biosurfactants was not observed in the SSB. In the SmgB, correlation coefficients of 91% and 87% were obtained between lipolytic activity and oil in water and water in oil emulsifying activities, respectively. A correlation of 84% was obtained between lipolytic activity and reduction of surface tension in the culture medium. The surface tension decreased from 50 to 28mNm(-1) indicating that biosurfactants were produced in the culture medium.
脂肪酶和生物表面活性剂是微生物产生的化合物，通常参与油脂底物的代谢。然而，脂肪酶和生物表面活性剂的产生之间的关系尚未确定。因此，本研究旨在评估从受柴油污染的土壤中分离出的曲霉属（Aspergillus spp.）在液态和固态生物过程（SmgB 和 SSB）中产生脂肪酶和生物表面活性剂之间的相关性。SSB 具有最高的脂肪酶产量，脂肪酶活性为 25.22U，而 SmgB 则为 4.52U。在 SSB 中未观察到生物表面活性剂的产生。在 SmgB 中，脂肪酶活性与水包油和油包水乳化活性之间的相关系数分别为 91%和 87%。脂肪酶活性与培养基中表面张力的降低之间的相关性为 84%。表面张力从 50 降低至 28mNm(-1)，表明在培养基中产生了生物表面活性剂。
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