Department of Organic Chemistry, Faculty of Science, University of Yaounde I, P.O. Box: 812, Yaounde, Cameroon.
Fitoterapia. 2010 Oct;81(7):873-7. doi: 10.1016/j.fitote.2010.05.011. Epub 2010 May 23.
Two new 5-methylcoumarin glycosides named diosfeboside A (1) and B (2) and five known compounds namely kaempferol 3-O-α-L-rhamnopyranosyl-(1 → 2)-β-D-glucopyranoside (3), ursolic acid (4), betulinic acid (5), stigmasterol (6) and stigmasterol 3-O-β-D-glucopyranoside (7) were isolated from the leaves of Diospyros crassiflora (Hiern). Their structures were established through interpretation of 1 and 2D NMR, mass spectra analysis and comparison with reported data. In vitro cytotoxic activity of the new compounds against human carcinoma cell lines (HL-60, Bel-7402, BGC-823, and KB) was evaluated and no cytotoxicity was observed for each of them.
从粗榧叶中分离得到两个新的 5-甲基香豆素糖苷，命名为 diosfeboside A（1）和 B（2），以及五个已知化合物，即山柰酚 3-O-α-L-鼠李吡喃糖基-(1 → 2)-β-D-吡喃葡萄糖苷（3）、熊果酸（4）、齐墩果酸（5）、豆甾醇（6）和豆甾醇 3-O-β-D-吡喃葡萄糖苷（7）。通过对 1 和 2D NMR、质谱分析和与文献数据的比较，确定了它们的结构。对新化合物进行了体外细胞毒性活性评价，对人癌细胞系（HL-60、Bel-7402、BGC-823 和 KB）进行了测试，结果显示每个化合物均无细胞毒性。
