诺维科夫肝癌细胞核抗原的纯化
Purification of nuclear antigens in Novikoff hepatoma.
作者信息
Fujitani H, Chiu J F, Hnilica L S
出版信息
Proc Natl Acad Sci U S A. 1978 Apr;75(4):1943-6. doi: 10.1073/pnas.75.4.1943.
Abstract
Nuclear antigen in Novikoff hepatoma chromatin was partially purified and characterized. As indicated by complement fixation assay, this antigen was present in chromatin of embryonic livers and several transplantable tumors. It was not detected in normal tissue chromatins of the same animals. For its immunological specificity this protein antigen (molecular weight 45,000-60,000) had to be complexed with DNA. Preliminary experiments indicate that specific nuclear protein antigens are also present in human tissues and spontaneous malignancies.
摘要
对诺维科夫肝癌染色质中的核抗原进行了部分纯化和特性鉴定。补体结合试验表明，这种抗原存在于胚胎肝脏和几种可移植肿瘤的染色质中。在同一动物的正常组织染色质中未检测到。就其免疫特异性而言，这种蛋白质抗原（分子量45,000 - 60,000）必须与DNA结合。初步实验表明，人组织和自发恶性肿瘤中也存在特异性核蛋白抗原。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
[Immunological studies on the antigenity of cell nucleus substances and on cell nucleus antibodies].
Z Rheumaforsch. 1962 Feb;21:13-20.
3
4
Immunological differences of deoxyribonucleoproteins from white blood cells of normal and leukaemic human beings.正常人和白血病患者白细胞中脱氧核糖核蛋白的免疫学差异
Nature. 1961 Jun 17;190:1122-3. doi: 10.1038/1901122b0.
5
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.用于比色法测定脱氧核糖核酸的二苯胺反应的条件及机制研究。
Biochem J. 1956 Feb;62(2):315-23. doi: 10.1042/bj0620315.
6
Nuclei from rat liver: isolation method that combines purity with high yield.大鼠肝脏细胞核：一种将纯度与高产率相结合的分离方法。
Science. 1966 Dec 30;154(3757):1662-5. doi: 10.1126/science.154.3757.1662.
7
Proteins of chromatin in template restriction. 3. The macromolecules in specific restriction of the chromatin DNA.模板限制中染色质的蛋白质。3. 染色质DNA特异性限制中的大分子。
Biochim Biophys Acta. 1971 Jan 28;228(2):550-62. doi: 10.1016/0005-2787(71)90061-x.
8
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
9
Sequence-specific interaction of DNA and chromosomal protein.DNA与染色体蛋白的序列特异性相互作用。
J Mol Biol. 1969 Jan;39(2):351-64. doi: 10.1016/0022-2836(69)90322-2.
10
Reversible denaturation of enzymes by sodium dodecyl sulfate.十二烷基硫酸钠对酶的可逆变性作用。
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