诺维科夫肝癌细胞核抗原的纯化
Purification of nuclear antigens in Novikoff hepatoma.
作者信息
Fujitani H, Chiu J F, Hnilica L S
出版信息
Proc Natl Acad Sci U S A. 1978 Apr;75(4):1943-6. doi: 10.1073/pnas.75.4.1943.
Abstract
Nuclear antigen in Novikoff hepatoma chromatin was partially purified and characterized. As indicated by complement fixation assay, this antigen was present in chromatin of embryonic livers and several transplantable tumors. It was not detected in normal tissue chromatins of the same animals. For its immunological specificity this protein antigen (molecular weight 45,000-60,000) had to be complexed with DNA. Preliminary experiments indicate that specific nuclear protein antigens are also present in human tissues and spontaneous malignancies.
摘要
对诺维科夫肝癌染色质中的核抗原进行了部分纯化和特性鉴定。补体结合试验表明，这种抗原存在于胚胎肝脏和几种可移植肿瘤的染色质中。在同一动物的正常组织染色质中未检测到。就其免疫特异性而言，这种蛋白质抗原（分子量45,000 - 60,000）必须与DNA结合。初步实验表明，人组织和自发恶性肿瘤中也存在特异性核蛋白抗原。
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