Sabouni Nasim, Mohammadi Mojgan, Boroumand Amir Reza, Palizban Sepideh, Tavakol Afshari Jalil
Department of Immunology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Iran J Basic Med Sci. 2024;27(9):1187-1196. doi: 10.22038/IJBMS.2024.74397.16197.
Enhancement of proliferation, pluripotency, and self-renewal capacity as the unique features of MSCs can improve their therapeutic potential to regenerate tissues. In this context, crocin and curcumin, carotenoid compounds with outstanding medicinal properties, could be promising for cell protection and growth. This study aimed to evaluate the impact of nanocurcumin and crocin on BM-MSCs proliferation and pluripotency .
BM-MSC were isolated from the iliac crest of SCI patients who were candidates for stem cell therapy. The effect of crocin and nanocurcumin on MSC proliferation was evaluated using MTT and PDT assay. The percentage of apoptotic MSCs was measured by flow cytometry. Furthermore, mRNA and protein expression of OCT4 and SOX2 as the proliferation and self-renewal related genes were quantified by real-time PCR and western blotting, respectively.
Our findings demonstrated that only low concentrations of nanocurcumin (0.3 and 0.7 µM) and crocin (2.5 5 µM) significantly affected MSCs proliferation and protected them from apoptosis. Also, crocin and nanocurcumin at low doses caused an elevation in the mRNA and protein expression levels of OCT4 and SOX2 genes. In contrast, high concentrations decreased the survival of MSCs and led to increased apoptosis compared with the untreated group.
Our results suggest that using nanocurcumin and crocin separately in culturing MSCs can be considered proliferative agents to prepare the more advantageous tool for cell therapies. However, more and preclinical research is needed in this area.
增强增殖、多能性和自我更新能力作为间充质干细胞的独特特征，可以提高其组织再生的治疗潜力。在这种情况下，西红花苷和姜黄素作为具有出色药用特性的类胡萝卜素化合物，可能对细胞保护和生长有前景。本研究旨在评估纳米姜黄素和西红花苷对骨髓间充质干细胞增殖和多能性的影响。
从干细胞治疗候选的脊髓损伤患者的髂嵴中分离出骨髓间充质干细胞。使用MTT和PDT试验评估西红花苷和纳米姜黄素对间充质干细胞增殖的影响。通过流式细胞术测量凋亡间充质干细胞的百分比。此外，分别通过实时PCR和蛋白质印迹法定量作为增殖和自我更新相关基因的OCT4和SOX2的mRNA和蛋白质表达。
我们的研究结果表明，只有低浓度的纳米姜黄素（0.3和0.7μM）和西红花苷（2.5和5μM）显著影响间充质干细胞的增殖并保护它们免于凋亡。此外，低剂量的西红花苷和纳米姜黄素导致OCT4和SOX2基因的mRNA和蛋白质表达水平升高。相比之下，高浓度降低了间充质干细胞的存活率并导致与未处理组相比凋亡增加。
我们的结果表明，在培养间充质干细胞时分别使用纳米姜黄素和西红花苷可以被认为是增殖剂，以制备用于细胞治疗的更有利工具。然而，该领域需要更多的临床前研究。
