Montilla R, Williams R P, Lorén J G, Viñas M
Departament de Microbiologia i Parasitologia Sanitàries, Facultat de Farmàcia, Universitat de Barcelona, Spain.
Antonie Van Leeuwenhoek. 1991 Jan;59(1):15-8. doi: 10.1007/BF00582114.
Kappa phage active on Serratia marcescens can form plaques on white and red strains with identical efficiencies. To identify the kappa phage receptor, the inactivation of the phage was studied after incubation with several bacterial subcellular fractions. The experiments demonstrated that kappa phage adsorbs to outer membrane fractions of susceptible cells. Proteinase K did not affect the rate of inactivation. Lipopolysaccharide proved to be the primary receptor for kappa phage. Prodigiosin content of the lipopolysaccharide fraction was low.
对粘质沙雷氏菌有活性的κ噬菌体能够以相同效率在白色菌株和红色菌株上形成噬菌斑。为了鉴定κ噬菌体受体，在与几种细菌亚细胞组分孵育后研究了噬菌体的失活情况。实验表明，κ噬菌体吸附到敏感细胞的外膜组分上。蛋白酶K不影响失活速率。脂多糖被证明是κ噬菌体的主要受体。脂多糖组分中灵菌红素的含量较低。
