Wang Shuyi, Yee Herman, Wen Hannah Y, Wang Beverly Y
Department of Pathology, Eye, Ear, Nose and Throat Hospital of Fudan University, Shanghai School of Medicine, Shanghai, China.
Head Neck Pathol. 2009 Sep;3(3):207-11. doi: 10.1007/s12105-009-0131-4. Epub 2009 Aug 2.
Squamous papilloma is a benign exophytic proliferation which can occur occasionally in the external ear canal. It is widely assumed that the Human Papilloma Virus (HPV) is an etiologic factor of papillomas. Available techniques for detection of HPV genomes include immunohistochemistry, Southern blot hybridization, in situ hybridization (ISH), and polymerase chain reaction. To our knowledge, HPV typing has not been reported on tissue sections of papillomas in the external ear canal. We report HPV ISH analysis in ten cases of papillomas, involving the external ear canal in Chinese patients. These papilloma excrescences were less than 1 cm in diameter, and were benign morphologically. Automated HPV ISH analysis was performed for the hybridization of DNA probes, including both low-risk and high-risk HPV subtypes. HPV ISH results revealed that seven out of ten cases were positive for low-risk HPV (6, 11), three cases demonstrated no hybridization for low-risk HPV probe, and none of the cases revealed any detection of high-risk HPV (16, 18, 31, 33, 35, 39, 51, 52, 56, 58, and 66). On follow-up after 18-29 months (average 24.5 months), eight patients were doing well, with no local recurrence after excision. Two patients were lost to follow-up. Our results confirm that benign papillomas of the external ear canal are associated with low-risk HPV infection with benign behavior and neither recurrence nor high grade dysplasia.
鳞状乳头状瘤是一种良性外生性增生，偶尔可发生于外耳道。人们普遍认为人乳头瘤病毒（HPV）是乳头状瘤的病因之一。现有的检测HPV基因组的技术包括免疫组织化学、Southern印迹杂交、原位杂交（ISH）和聚合酶链反应。据我们所知，外耳道乳头状瘤组织切片的HPV分型尚未见报道。我们报告了对10例中国患者外耳道乳头状瘤的HPV ISH分析。这些乳头状瘤赘生物直径小于1厘米，形态学上为良性。对包括低风险和高风险HPV亚型在内的DNA探针进行自动化HPV ISH杂交分析。HPV ISH结果显示，10例中有7例低风险HPV（6、11）呈阳性，3例低风险HPV探针未杂交，所有病例均未检测到高风险HPV（16、18、31、33、35、39、51、52、56、58和66）。在18 - 29个月（平均24.5个月）的随访中，8例患者情况良好，切除后无局部复发。2例患者失访。我们的结果证实，外耳道良性乳头状瘤与低风险HPV感染相关，行为良性，无复发及高级别发育异常。
