Department of Microbiology, College of Medicine and Medical Research Institute, Chungbuk National University, 12 Gaeshin-Dong, Heungduk-Ku, Cheongju, Chungbuk, South Korea.
Virus Res. 2010 Sep;152(1-2):137-52. doi: 10.1016/j.virusres.2010.06.018. Epub 2010 Jun 25.
We have determined the complete nucleotide and deduced amino acid sequences of the RNA genome of CBNU1, a human norovirus (NoV) recovered from a 2006 outbreak in South Korea. The genome of 7547 nucleotides, excluding a 3'-poly(A) tail of 11-105 nucleotides, encodes three overlapping open reading frames (ORFs): ORF1 (nucleotides 5-5104), ORF2 (nucleotides 5085-6731), and ORF3 (nucleotides 6731-7495). In a comparison to 108 other currently available completely sequenced NoVs representing all five genogroups (GI-GV) except GIV, the CBNU1 strain was highly similar to GII.3 NoVs. Multiple sequence alignments of the completely sequenced NoV genomes revealed five hypervariable regions throughout their genomes: two in ORF1, one in ORF2, and two in ORF3. At both the nucleotide and amino acid levels, genome-based phylogenetic analyses invariably showed that the CBNU1 strain was most closely related to three GII.3 NoVs: the American Texas/TCH04-577 and the two Japanese Saitama U18 and Saitama U201 strains; furthermore, these genome-based phylogenetic topologies corresponded most closely to those based on the ORF2 genes, as compared to those based on the ORF1 and ORF3 genes. Subsequent ORF2-based phylogenetic analyses of a selection of 126 other NoVs representing all 19 GII genotypes, in combination with genome-based Simplot analyses, showed that the CBNU1 strain was a recombinant GII.3 NoV with a breakpoint at the ORF1/ORF2 junction between two putative parent-like strains, Guangzhou/NVgz01 and Texas/TCH04-577. Overall, the CBNU1 strain represents the first Korean human NoV whose genome has been completely sequenced and for which its relationship with a large panel of genetically diverse NoVs has been extensively characterized.
我们确定了韩国 2006 年爆发疫情中分离的人诺如病毒（NoV）CBNU1 的完整 RNA 基因组的核苷酸和推导的氨基酸序列。该基因组长 7547 个核苷酸，不包括 3'-聚（A）尾的 11-105 个核苷酸，编码三个重叠的开放阅读框（ORF）：ORF1（核苷酸 5-5104）、ORF2（核苷酸 5085-6731）和 ORF3（核苷酸 6731-7495）。与代表所有五个基因组群（GI-GV）的 108 个其他现有完全测序的 NoV 相比，CBNU1 株与 GII.3 NoV 高度相似。对完全测序的 NoV 基因组的多序列比对显示，其基因组中有五个高变区：ORF1 中有两个，ORF2 中有一个，ORF3 中有两个。在核苷酸和氨基酸水平上，基于基因组的系统发育分析始终表明，CBNU1 株与三个 GII.3 NoV 最密切相关：美国德克萨斯州/TCH04-577 株和日本的两个埼玉 U18 和埼玉 U201 株；此外，与基于 ORF1 和 ORF3 基因的系统发育拓扑结构相比，这些基于基因组的系统发育拓扑结构与基于 ORF2 基因的系统发育拓扑结构最接近。随后对代表所有 19 个 GII 基因型的 126 个其他 NoV 的基于 ORF2 的系统发育分析，结合基于基因组的 Simplot 分析，表明 CBNU1 株是一种重组 GII.3 NoV，其断点位于两个假定亲本样株 Guangzhou/NVgz01 和 Texas/TCH04-577 之间的 ORF1/ORF2 交界处。总体而言，CBNU1 株代表了第一个完全测序的韩国人 NoV，并且其与大量遗传多样的 NoV 的关系已得到广泛描述。
