Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai JiaoTong University, 800 Dongchuan Road, Shanghai, People's Republic of China.
Mol Biol Rep. 2012 Feb;39(2):1275-81. doi: 10.1007/s11033-011-0845-8. Epub 2011 May 25.
Noroviruses (NoVs) are one of the major causal agents of acute gastroenteritis in both industrial and developing countries including China. Recent studies have revealed that NoV genome is highly prone to mutation and recombination which may lead to emergence of new strains. In the present study, three full-length genomes of human NoV from China were determined and the genomic organization and recombination were analyzed. They had similar genome organization and contained three predicted ORFs, though the 5'UTR of those three strains were 2, 4 and 8 nucleotides, respectively. Phylogenetic analysis showed that the HU/GII/SHANGHAI/SH312/2008/CHN strain may be a recombinant of GII-3 capsid and GII-4 polymerase. To confirm the finding and detect the breakpoints where the recombination event occurred, we performed recombination analysis based on the genomic sequences of HU/GII/SHANGHAI/SH312/2008/CHN as the query sequence, and AB220921/NOV/JP/GII-4 and AB365435/NOV/US/GII-3 as the background sequences, using RPD software. Results indicated that the two parental strains were AB220921/NOV/JP/GII-4 and AB365435/NOV/US/GII-3. The breakpoint for this recombination event located at position 5,107 nt of the genome (in the ORF1 and ORF2 overlap).
诺如病毒（NoVs）是包括中国在内的工业和发展中国家急性肠胃炎的主要病原体之一。最近的研究表明，NoV 基因组极易发生突变和重组，这可能导致新菌株的出现。在本研究中，确定了来自中国的三种人类诺如病毒的全长基因组，并分析了基因组组织和重组。它们具有相似的基因组组织，包含三个预测的 ORF，尽管这三个菌株的 5'UTR 分别为 2、4 和 8 个核苷酸。系统发育分析表明，HU/GII/SHANGHAI/SH312/2008/CHN 株可能是 GII-3 衣壳和 GII-4 聚合酶的重组体。为了证实这一发现并检测重组事件发生的断点，我们使用 RPD 软件，以 HU/GII/SHANGHAI/SH312/2008/CHN 作为查询序列的基因组序列为基础，对 AB220921/NOV/JP/GII-4 和 AB365435/NOV/US/GII-3 作为背景序列进行了重组分析。结果表明，两个亲本株分别为 AB220921/NOV/JP/GII-4 和 AB365435/NOV/US/GII-3。该重组事件的断点位于基因组的 5107nt 位置（ORF1 和 ORF2 重叠处）。
