Dukanovic Jovana, Rapaport Doron
Interfaculty Institute for Biochemistry, Hoppe-Seyler-Str. 4, University of Tübingen, 72076 Tübingen, Germany.
Biochim Biophys Acta. 2011 Mar;1808(3):971-80. doi: 10.1016/j.bbamem.2010.06.021. Epub 2010 Jun 30.
Proteins residing in the mitochondrial outer membrane facilitate various interactions between the organelle and the rest of the eukaryotic cell. All these proteins are encoded in the nucleus and synthesized on cytosolic ribosomes. Thus, they have to bear appropriate signals that ensure both their correct import into the organelle and their ability to acquire different topologies in the lipid bilayer. None of these proteins contain a canonical cleavable N-terminal presequence. Rather, the targeting and sorting signals can be found at their termini or in internal structural elements. In this review, we summarize the current knowledge regarding the diverse molecular mechanisms by which mitochondrial outer membrane proteins are specifically targeted to the organelle and inserted into the target membrane. Recognition events in the diverse pathways and the driving force for the various stages of this process will be discussed. This article is part of a Special Issue entitled Protein translocation across or insertion into membranes.
位于线粒体外膜的蛋白质促进了该细胞器与真核细胞其他部分之间的各种相互作用。所有这些蛋白质都在细胞核中编码，并在胞质核糖体上合成。因此，它们必须带有适当的信号，以确保它们正确导入细胞器，并确保它们在脂质双层中获得不同拓扑结构的能力。这些蛋白质均不包含典型的可切割N端前序列。相反，靶向和分选信号可以在它们的末端或内部结构元件中找到。在这篇综述中，我们总结了目前关于线粒体外膜蛋白特异性靶向细胞器并插入目标膜的多种分子机制的知识。将讨论不同途径中的识别事件以及该过程各个阶段的驱动力。本文是名为“蛋白质跨膜转运或插入膜”的特刊的一部分。
