Laboratory for Biomechanics and Biomaterials, Department of Orthopaedic Surgery, Hannover Medical School, Anna-von-Borries-Straße 1-7, 30625 Hannover, Germany.
Acta Biomater. 2010 Nov;6(11):4447-55. doi: 10.1016/j.actbio.2010.06.022. Epub 2010 Jun 25.
Calcified tissues, like bones and teeth, are among the most challenging tissues for histological research. However, especially with respect to dental or orthopaedic research, powerful histological techniques are necessary to study pathological conditions or traumatic injuries, and to investigate the molecular and cellular mechanisms of regeneration processes and functional recovery. The situation is even more complicated in orthopaedic research because here metallic implants or other devices made of various materials are often present, and the hard tissue-implant interface is of crucial interest in both biocompatibility and functional recovery research. After the cutting-grinding technique, embedding in technical resins is the most promising approach. Here we describe an optimized and standardized embedding and cutting technique using Technovit 9100 New. Using this technique, we are able to perform enzyme histochemistry, immunohistochemistry, a great variety of classical histological stains and even in situ hybridization.
钙化组织，如骨骼和牙齿，是组织学研究中最具挑战性的组织之一。然而，特别是在牙科或骨科研究方面，强大的组织学技术对于研究病理状况或创伤损伤、研究再生过程和功能恢复的分子和细胞机制是必要的。在骨科研究中情况更加复杂，因为这里经常存在金属植入物或其他由各种材料制成的装置，而硬组织-植入物界面在生物相容性和功能恢复研究中至关重要。在切割-研磨技术之后，用技术树脂进行包埋是最有前途的方法。在这里，我们描述了一种使用 Technovit 9100 New 的优化和标准化的包埋和切割技术。使用该技术，我们能够进行酶组织化学、免疫组织化学、多种经典组织学染色，甚至原位杂交。
