Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch, Russian Academy of Sciences, 690022 Vladivostok, Prospect 100 Let Vladivostoku, 159, Russia.
NODAI Culture Collection Center, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
Int J Syst Evol Microbiol. 2011 Jun;61(Pt 6):1375-1381. doi: 10.1099/ijs.0.026047-0. Epub 2010 Jul 2.
An aerobic, Gram-stain-negative, non-pigmented, non-motile bacterium, strain KMM 9031(T), was isolated from a sandy sediment sample collected from the shore of the Sea of Japan and subjected to phenotypic and phylogenetic analysis. Based on comparative 16S rRNA gene sequence analysis, strain KMM 9031(T) constituted a separate phylogenetic line within the Roseobacter clade of the class Alphaproteobacteria, sharing highest sequence similarities with members of the genera Roseovarius (92.7-95.3 %), Pseudoruegeria (94.5 %), Sulfitobacter (92.7-94.4 %) and Thalassobacter (94.2-94.3 %). The predominant fatty acid of strain KMM 9031(T) was C(18 : 1)ω7c, with C(16 : 0), C(10 : 0) 3-OH and C(12 : 1) 3-OH present in lesser amounts. The DNA G+C content of the isolate was 52.6 mol%. The major isoprenoid quinone was Q-10 and polar lipids comprised phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol and two unknown lipids. On the basis of phylogenetic analysis and physiological and biochemical characterization, strain KMM 9031(T) represents a novel species in a new genus, for which the name Pacificibacter maritimus gen. nov., sp. nov. is proposed; the type strain is KMM 9031(T) ( = NRIC 0785(T) = JCM 17096(T)).
一株好氧、革兰氏阴性、非色素、非运动的细菌，菌株 KMM 9031(T)，从日本海岸边的沙质沉积物样本中分离出来，并进行了表型和系统发育分析。基于比较 16S rRNA 基因序列分析，菌株 KMM 9031(T)在 α-变形菌纲的玫瑰杆菌群中构成了一个单独的系统发育分支，与玫瑰杆菌属（92.7-95.3%）、假交替单胞菌属（94.5%）、硫杆菌属（92.7-94.4%）和海洋杆菌属（94.2-94.3%）的成员具有最高的序列相似性。菌株 KMM 9031(T)的主要脂肪酸是 C(18 : 1)ω7c，其次是 C(16 : 0)、C(10 : 0) 3-OH 和 C(12 : 1) 3-OH。该分离株的 DNA G+C 含量为 52.6 mol%。主要的异戊二烯醌是 Q-10，极性脂类包括磷脂酰胆碱、磷脂酰甘油、双磷脂酰甘油和两种未知脂类。基于系统发育分析和生理生化特征，菌株 KMM 9031(T)代表了一个新属中的一个新种，建议将其命名为太平洋海杆菌属 nov. gen. nov.，sp. nov.；其模式株是 KMM 9031(T)（=NRIC 0785(T)=JCM 17096(T)）。
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