Bull World Health Organ. 1965;32(1):47-58.
Studies on the development of encephalitogenic activity in the cerebral tissue of various animals (rabbits, rats, mice and sheep) showed that the brains of albino rats did not become encephalitogenic until after the 18th day of life, which is later than in any of the other animals studied. On the basis of this finding, a method was developed for the preparation of an entirely allergen-free, non-encephalitogenic antirabies vaccine using the brains of suckling rats. The phenolized vaccine, both in liquid and in lyophilized form, consistently gave high antigenic titres when tested in animals and produced a good increase in virus-neutralizing antibodies in man. It also showed a low thromboplastic activity. More than 1500 litres of this vaccine have since been manufactured on an industrial scale and more than 9500 persons vaccinated. General reactions have been far less frequent than with the conventional Fermi vaccine and no neuroparalytic accidents or shock reactions have been reported. Vaccination with the allergen-free vaccine has proceeded smoothly even in persons considered to be especially at risk owing to previous vaccination with antirabies vaccine or a history of trauma or disease of the central nervous system.
对各种动物（兔、鼠、鼠和羊）脑组织致脑炎活性的研究表明，白化鼠的脑组织在出生后第 18 天之前不会产生致脑炎活性，这比其他任何研究的动物都晚。基于这一发现，开发了一种使用乳鼠脑组织制备完全无过敏原、非致脑炎性狂犬病疫苗的方法。用苯酚处理的疫苗，无论是液态还是冻干形式，在动物试验中均具有较高的抗原效价，并在人体中产生了良好的病毒中和抗体增加。它还显示出较低的血栓形成活性。此后，已在工业规模上生产了这种疫苗超过 1500 升，并对 9500 多人进行了接种。一般反应比传统的 Fermi 疫苗少得多，也没有报告神经麻痹事故或休克反应。即使对于那些由于先前接种狂犬病疫苗或中枢神经系统创伤或疾病而被认为特别处于危险之中的人，使用无过敏原疫苗进行接种也进行得很顺利。
