实验室规模的蛋白质条带系统，用于将生物分子图案化到基于纸张的免疫层析测试条上。
Laboratory-scale protein striping system for patterning biomolecules onto paper-based immunochromatographic test strips.
机构信息
University of Washington, Department of Bioengineering, Box 355061, 3720 15th Ave NE, Seattle, WA 98195-5061, USA.
出版信息
Lab Chip. 2010 Sep 7;10(17):2279-82. doi: 10.1039/c004991c. Epub 2010 Jul 6.
Abstract
A method for patterning narrow lines of biomolecules onto nitrocellulose membranes using laboratory syringe pumps is described. One syringe pump is used to drive the biomolecule solution through a needle, while another modified syringe pump acts as a one-dimensional translation stage, moving the needle across the membrane much like a pen. This method consumes very small volumes of reagents, and is a viable option for laboratory-scale fabrication and prototyping of point-of-care rapid diagnostic test strips.
摘要
介绍了一种使用实验室注射泵将生物分子图案化到硝酸纤维素膜上的方法。一个注射器泵用于驱动生物分子溶液通过针头，而另一个改装的注射器泵则充当一维平移台，使针头像笔一样在膜上移动。这种方法消耗的试剂非常少，是在实验室规模上制造和原型制作即时诊断测试条的可行选择。
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