beòcarta Ltd., Royal College Building, Glasgow, UK.
Lett Appl Microbiol. 2010 Sep;51(3):252-7. doi: 10.1111/j.1472-765X.2010.02890.x. Epub 2010 Jun 17.
Aspergillus terreus produces itaconic acid at low pH but lovastatin and other secondary metabolites at higher pH in the fermentation. The utilization of glucose as a carbon substrate was investigated for secondary metabolite production by A. terreus.
With a starting pH of 6.5, glucose was rapidly metabolized to gluconic acid by the wild-type strain and by transformants harbouring Aspergillus niger genes encoding 6-phosphofructo-1-kinases with superior kinetic and regulatory properties for bioproduction of metabolites from glucose. On exhaustion of the glucose in batch fermentations, the accumulated gluconic acid was utilized as a carbon source.
A novel pathway of glucose catabolism was demonstrated in A. terreus, a species whose wild type is, without any strain development, capable of producing gluconic acid at high molar conversion efficiency (up to 0.7 mol mol(-1) glucose consumed).
Aspergillus terreus is a potential novel producer organism for gluconic acid, a compound with many uses as a bulk chemical. With a new knowledge of glucose catabolism by A. terreus, fermentation strategies for secondary metabolite production can be devised with glucose feeding using feedback regulation by pH.
土曲霉在低 pH 值下产生衣康酸，但在发酵过程中在较高 pH 值下产生洛伐他汀和其他次级代谢物。本研究旨在探讨葡萄糖作为碳源对土曲霉次级代谢产物生产的利用。
以起始 pH 值 6.5 进行发酵，野生型菌株和携带编码具有生物生产代谢产物的葡萄糖激酶的黑曲霉基因的转化体迅速将葡萄糖代谢为葡萄糖酸。在分批发酵中葡萄糖耗尽后，积累的葡萄糖酸被用作碳源。
在土曲霉中证明了一种新的葡萄糖分解代谢途径，该物种的野生型在没有任何菌株开发的情况下，能够以高摩尔转化率（高达 0.7 mol mol(-1) 消耗的葡萄糖）生产葡萄糖酸。
土曲霉是一种有潜力的新型葡萄糖酸生产菌，葡萄糖酸是一种具有多种用途的大宗化学品。通过了解土曲霉的葡萄糖分解代谢，我们可以设计使用 pH 反馈调节进行葡萄糖补料的次级代谢产物发酵策略。
