鉴定三种铜绿微囊藻收集株中的微囊藻毒素。
Identification of microcystins from three collection strains of Microcystis aeruginosa.
机构信息
Departamento de Biología, Universidad Autónoma de Madrid, Cantoblanco, 28049-Madrid, Spain.
出版信息
Environ Pollut. 2010 Sep;158(9):2906-14. doi: 10.1016/j.envpol.2010.06.018.
Microcystins (MCs) are toxic cyclic heptapeptides produced by various cyanobacteria genera, especially Microcystis. We identified 10 out of 12 MCs produced by three Microcystis aeruginosa strains from cyanobacteria collections, UTEX 2666, UTEX 2670 and UAM 1303, by using two analytical methods: Matrix-assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF/MS) and HPLC Photodiode Array Detector coupled to a hybrid Quadrupole Time of Flight Mass Spectrometry (HPLC-PDA-QTOF/MS). MALDI-TOF/MS failed to detect non-polar MCs, such as MC-LY and MC-LW. HPLC-QTOF/MS permitted the accurate identification of most MCs present in methanolic extracts. Besides, three new MCs, namely: [D-Glu(OCH3)6, D-Asp3] MC-LAba, MC-YL and MC-YM were detected by HPLC-QTOF/MS.
微囊藻毒素（MCs）是由各种蓝藻属产生的有毒环状七肽，尤其是微囊藻。我们使用两种分析方法：基质辅助激光解吸电离飞行时间质谱（MALDI-TOF/MS）和高效液相色谱光电二极管阵列检测器与混合四极杆飞行时间质谱（HPLC-PDA-QTOF/MS），从蓝藻收藏物 UTEX 2666、UTEX 2670 和 UAM 1303 中的三个铜绿微囊藻菌株中鉴定出 12 种 MC 中的 10 种。MALDI-TOF/MS 无法检测非极性 MC，如 MC-LY 和 MC-LW。HPLC-QTOF/MS 允许准确鉴定甲醇提取物中存在的大多数 MC。此外，通过 HPLC-QTOF/MS 检测到三种新的 MC，分别为：[D-Glu（OCH3）6，D-Asp3] MC-LAba、MC-YL 和 MC-YM。
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