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通过Alu-PCR产物的直接杂交将辐射杂种定位到黏粒和酵母人工染色体文库。

Mapping irradiation hybrids to cosmid and yeast artificial chromosome libraries by direct hybridization of Alu-PCR products.

作者信息

Monaco A P, Lam V M, Zehetner G, Lennon G G, Douglas C, Nizetic D, Goodfellow P N, Lehrach H

机构信息

Genome Analysis Laboratory, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London, UK.

出版信息

Nucleic Acids Res. 1991 Jun 25;19(12):3315-8. doi: 10.1093/nar/19.12.3315.

DOI:10.1093/nar/19.12.3315
PMID:2062647
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC328328/
Abstract

A direct hybridization protocol is described for screening cosmid and yeast artificial chromosome libraries with pools of Alu-PCR products from somatic cell or irradiation hybrids. This method eliminates purification, cloning and analysis of each individual Alu-PCR product before library screening. A series of human X chromosome irradiation hybrids were mapped by this method, using a cosmid reference library for comparisons between overlapping hybrids to identify interesting clones for further analysis.

摘要

本文描述了一种直接杂交方案，用于用来自体细胞或辐射杂种的Alu-PCR产物池筛选黏粒文库和酵母人工染色体文库。该方法无需在文库筛选前对每个单独的Alu-PCR产物进行纯化、克隆和分析。利用该方法对一系列人类X染色体辐射杂种进行了定位，使用黏粒参考文库在重叠杂种之间进行比较，以鉴定出有趣的克隆进行进一步分析。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cce3/328328/1f1a5764d3cc/nar00092-0135-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cce3/328328/e6a534ba8480/nar00092-0135-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cce3/328328/3dd58b805bf9/nar00092-0135-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cce3/328328/1f1a5764d3cc/nar00092-0135-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cce3/328328/e6a534ba8480/nar00092-0135-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cce3/328328/3dd58b805bf9/nar00092-0135-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cce3/328328/1f1a5764d3cc/nar00092-0135-c.jpg

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本文引用的文献

1
"A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.一种将DNA限制性内切酶片段放射性标记至高比活度的技术。附录
Anal Biochem. 1984 Feb;137(1):266-7. doi: 10.1016/0003-2697(84)90381-6.
2
Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors.通过人工染色体载体将大片段外源DNA克隆到酵母中。
Science. 1987 May 15;236(4803):806-12. doi: 10.1126/science.3033825.
3
A deletion hot spot in the Duchenne muscular dystrophy gene.杜氏肌营养不良基因中的一个缺失热点。
一张整合了酵母人工染色体（YACs）、基因和序列标签位点（STS）标记的覆盖整个人类X染色体的辐射杂种图谱。
Mamm Genome. 1996 Oct;7(10):758-66. doi: 10.1007/s003359900227.
4
Efficient identification and regional positioning of YAC and cosmid clones to human chromosome 21 by radiation fusion hybrids.通过辐射杂种细胞将YAC和黏粒克隆高效鉴定并定位到人类21号染色体上。
Mamm Genome. 1994 Jun;5(6):365-71. doi: 10.1007/BF00356556.
5
Characterization of microsatellites from flow-sorted porcine chromosome 13.来自流式分选猪13号染色体的微卫星特征分析。
Mamm Genome. 1994 Nov;5(11):707-11. doi: 10.1007/BF00426077.
6
Irradiation and fusion gene transfer.辐射与融合基因转移。
Mol Biotechnol. 1995 Apr;3(2):117-28. doi: 10.1007/BF02789107.
7
W (A or T) sequences as probes and primers suitable for genomic mapping and fingerprinting.作为适用于基因组作图和指纹分析的探针及引物的W（A或T）序列。
Nucleic Acids Res. 1991 Nov 11;19(21):5839-42. doi: 10.1093/nar/19.21.5839.
8
A two-dimensional YAC pooling strategy for library screening via STS and Alu-PCR methods.一种通过STS和Alu-PCR方法进行文库筛选的二维酵母人工染色体（YAC）池化策略。
Nucleic Acids Res. 1992 May 25;20(10):2559-63. doi: 10.1093/nar/20.10.2559.
Genomics. 1988 Feb;2(2):101-8. doi: 10.1016/0888-7543(88)90090-0.
4
Construction and screening of a genomic library specific for mouse chromosome 16.小鼠16号染色体特异性基因组文库的构建与筛选
Proc Natl Acad Sci U S A. 1989 Nov;86(21):8482-6. doi: 10.1073/pnas.86.21.8482.
5
Alu polymerase chain reaction: a method for rapid isolation of human-specific sequences from complex DNA sources.Alu聚合酶链式反应：一种从复杂DNA来源中快速分离人特异性序列的方法。
Proc Natl Acad Sci U S A. 1989 Sep;86(17):6686-90. doi: 10.1073/pnas.86.17.6686.
6
A method for generating hybrids containing nonselected fragments of human chromosomes.一种生成包含人类染色体非选择片段的杂种细胞的方法。
Genomics. 1989 May;4(4):509-17. doi: 10.1016/0888-7543(89)90274-7.
7
Segregation of the Huntington disease region of human chromosome 4 in a somatic cell hybrid.人类4号染色体亨廷顿病区域在一个体细胞杂种中的分离
Genomics. 1989 Apr;4(3):397-407. doi: 10.1016/0888-7543(89)90347-9.
8
Cloning the gene for an inherited human disorder--chronic granulomatous disease--on the basis of its chromosomal location.基于染色体定位克隆一种遗传性人类疾病——慢性肉芽肿病——的基因。
Nature. 1986;322(6074):32-8. doi: 10.1038/322032a0.
9
Rapid isolation of human chromosome-specific DNA probes from a somatic cell hybrid.从体细胞杂种中快速分离人染色体特异性DNA探针。
Genomics. 1990 Jun;7(2):257-63. doi: 10.1016/0888-7543(90)90548-9.
10
Rapid isolation of DNA probes within specific chromosome regions by interspersed repetitive sequence polymerase chain reaction.通过散布重复序列聚合酶链反应快速分离特定染色体区域内的DNA探针。
Genomics. 1990 Mar;6(3):475-81. doi: 10.1016/0888-7543(90)90477-c.