通过Alu-PCR产物的直接杂交将辐射杂种定位到黏粒和酵母人工染色体文库。
Mapping irradiation hybrids to cosmid and yeast artificial chromosome libraries by direct hybridization of Alu-PCR products.
作者信息
Monaco A P, Lam V M, Zehetner G, Lennon G G, Douglas C, Nizetic D, Goodfellow P N, Lehrach H
机构信息
Genome Analysis Laboratory, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London, UK.
出版信息
Nucleic Acids Res. 1991 Jun 25;19(12):3315-8. doi: 10.1093/nar/19.12.3315.
A direct hybridization protocol is described for screening cosmid and yeast artificial chromosome libraries with pools of Alu-PCR products from somatic cell or irradiation hybrids. This method eliminates purification, cloning and analysis of each individual Alu-PCR product before library screening. A series of human X chromosome irradiation hybrids were mapped by this method, using a cosmid reference library for comparisons between overlapping hybrids to identify interesting clones for further analysis.
本文描述了一种直接杂交方案，用于用来自体细胞或辐射杂种的Alu-PCR产物池筛选黏粒文库和酵母人工染色体文库。该方法无需在文库筛选前对每个单独的Alu-PCR产物进行纯化、克隆和分析。利用该方法对一系列人类X染色体辐射杂种进行了定位，使用黏粒参考文库在重叠杂种之间进行比较，以鉴定出有趣的克隆进行进一步分析。
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