通过辐射杂种细胞将YAC和黏粒克隆高效鉴定并定位到人类21号染色体上。
Efficient identification and regional positioning of YAC and cosmid clones to human chromosome 21 by radiation fusion hybrids.
作者信息
Kumlien J, Labella T, Zehetner G, Vatcheva R, Nizetic D, Lehrach H
机构信息
Department of Genome Analysis, Imperial Cancer Research Fund, London, UK.
出版信息
Mamm Genome. 1994 Jun;5(6):365-71. doi: 10.1007/BF00356556.
The use of integrated mapping strategies involving bacterial, yeast, and rodent cells as hosts simplifies the construction of maps, which combine long-range order, high resolution, and easy access to the cloned DNA. Radiation-fusion hybrids offer a specially powerful long-range mapping system for human chromosomes. We describe here techniques for establishing a radiation-fusion hybrid map of Chromosome (Chr) 21q and its integration with local information on YAC and cosmid positions.
使用涉及细菌、酵母和啮齿动物细胞作为宿主的综合作图策略，简化了图谱的构建，这些图谱结合了长程有序性、高分辨率以及对克隆DNA的便捷获取。辐射杂种细胞为人类染色体提供了一种特别强大的长程作图系统。我们在此描述建立21号染色体长臂（Chr 21q）辐射杂种细胞图谱及其与酵母人工染色体（YAC）和黏粒位置的局部信息整合的技术。
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