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一种用于检测微藻中高粘性胞外聚合物的新工具。

A new tool to detect high viscous exopolymers from microalgae.

机构信息

Laboratoire de Génie Chimique et Biochimique, Clermont Université, Université Blaise Pascal, Polytech' Clermont Ferrand, 24 avenue des Landais, Aubière Cedex, France.

出版信息

J Ind Microbiol Biotechnol. 2011 Feb;38(2):319-26. doi: 10.1007/s10295-010-0775-9. Epub 2010 Jul 16.

Abstract

Microalgae are microorganisms often surrounded by a slime layer made of secreted polymeric substances sometimes including polysaccharides. These polysaccharides, weakly described in the literature, can constitute value-added molecules in several industrial areas. The aim of this article is to show that a new tool, the BioFilm Ring Test®, can be used to detect viscous microalgal exopolymers. Two red microalgal strains (Rhodella violacea and Porphyridium purpureum), one cyanobacterium (Arthrospira platensis) and their excreted polymeric fractions were studied. R. violacea and P. purpureum induced a positive response with the BioFilm Ring Test® contrary to A. platensis. Finally, the understanding of the fractions viscosity involvement in the BRT response was performed by a rheological study.

摘要

微藻是一种微生物,通常被一层由分泌的聚合物质组成的黏液层所包围,这些聚合物质有时包括多糖。这些多糖在文献中被描述得很弱,但可以在几个工业领域构成增值分子。本文的目的是表明,一种新工具,生物膜环试验(BioFilm Ring Test®),可以用于检测粘性微藻胞外聚合物。两种红色微藻(Rhodella violacea 和 Porphyridium purpureum)、一种蓝藻(Arthrospira platensis)及其分泌的聚合部分进行了研究。R. violacea 和 P. purpureum 与 A. platensis 相反,用 BioFilm Ring Test® 诱导出阳性反应。最后,通过流变学研究来研究部分粘性在 BRT 反应中的作用。

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