School of Biomedical Sciences, University of Queensland, St Lucia, Brisbane, QLD, Australia.
Int J Biochem Cell Biol. 2010 Oct;42(10):1586-9. doi: 10.1016/j.biocel.2010.07.006. Epub 2010 Jul 15.
Pancreatic acinar cells secrete fluid and digestive enzymes. Both types of secretion are activated by a rise in intracellular calcium but how the stimulus-secretion cascade actually regulates secretory output is not well understood. It has long been known that the calcium response of acinar cells to physiological stimulation is complex. Dependent on the type and concentration of agonist, it consists of either local or global calcium increases as well as spreading waves of calcium across the cell. In the past it has been speculated that these different calcium signals drive different secretory responses. Now, recent employment of two-photon microscopy has enabled the simultaneous recording of both enzyme secretion and calcium signals and is beginning to resolve this issue. The data shows that local calcium responses exclusively drive fluid secretion. Where-as, global calcium responses drive both fluid and enzyme secretion. This differential control of secretory output is likely central to controlling the physiological responses of pancreatic acinar cells.
胰腺腺泡细胞分泌液体和消化酶。这两种分泌类型都被细胞内钙离子浓度的上升所激活，但刺激-分泌级联实际上如何调节分泌输出还不是很清楚。长期以来，人们都知道胰腺腺泡细胞对生理刺激的钙反应是复杂的。取决于激动剂的类型和浓度，它包括局部或全局的钙增加以及钙在细胞内的传播波。过去，人们推测这些不同的钙信号驱动不同的分泌反应。现在，双光子显微镜的最新应用使得同时记录酶分泌和钙信号成为可能，并开始解决这个问题。数据显示，局部钙反应专门驱动液体分泌。而全局钙反应则同时驱动液体和酶的分泌。这种分泌输出的差异控制可能是控制胰腺腺泡细胞生理反应的关键。
