Protective effects of kappa-ca3000+CP against ultraviolet-induced damage in HaCaT and MEF cells.

In this study, the complex kappa-ca3000+CP combined collagen peptide with kappa-carrageenan oligosaccharide was tested for its ability to moderate UV-induced damage and investigated for its protective mechanism against UV radiation. Human keratinocytes (HaCaT) and mouse embryonic fibroblasts (MEF) were used to monitor the effects of kappa-ca3000+CP on cell viability, apoptosis, level of collagen I and MMP-1, MAPKs activation and intracellular ROS production after UV-irradiation. The results indicated that application of the kappa-ca3000+CP (100 microg/ml) could significantly attenuate UV-induced HaCaT and MEF death, as well as inhibit the UV-induced apoptosis of HaCaT cells. The decreased collagen I synthesis and the increased MMP-1 expression of MEF by UV radiation were almost restored back to normal level after treatment with kappa-ca3000+CP. Moreover, kappa-ca3000+CP could significantly suppress UV-induced MAPKs activation and intracellular ROS production. Taken together, these results showed that antioxidant property of kappa-ca3000+CP can effectively attenuate UV-caused cell damage and skin photoaging by suppressing cell apoptosis and expression of MMP-1 through the MAPKs signaling pathways. Thus, kappa-ca3000+CP has potential antiaging effects and prominent protective effects on UV-induced skin cell damages, which might be used in pharmaceutical and cosmetic industries.