Medical College of Georgia, Department of Orthopaedic Surgery, 1459 Laney Walker Blvd., Augusta, GA 30912, USA.
Bone. 2010 Oct;47(4):746-55. doi: 10.1016/j.bone.2010.07.014. Epub 2010 Jul 16.
Recent studies suggest that whole-body vibration (WBV) can improve measures of bone health for certain clinical conditions and ages. In the elderly, there also is particular interest in assessing the ability of physical interventions such as WBV to improve coordination, strength, and movement speed, which help prevent falls and fractures and maintain ambulation for independent living. The current study evaluated the efficacy of WBV in an aging mouse model. Two levels of vibration--0.5 and 1.5g--were applied at 32Hz to CB57BL/6 male mice (n=9 each) beginning at age 18 months and continuing for 12 weeks, 30 min/day, in a novel pivoting vibration device. Previous reports indicate that bone parameters in these mice begin to decrease substantially at 18 months, equivalent to mid-fifties for humans. Micro-computed tomography (micro-CT) and biomechanical assessments were made in the femur, radius, and lumbar vertebra to determine the effect of these WBV magnitudes and durations in the aging model. Sera also were collected for analysis of bone formation and breakdown markers. Mineralizing surface and cell counts were determined histologically. Bone volume in four regions of the femur did not change significantly, but there was a consistent shift toward higher mean density in the bone density spectrum (BDS), with the two vibration levels producing similar results. This new parameter represents an integral of the conventional density histogram. The amount of high density bone statistically improved in the head, neck, and diaphysis. Biomechanically, there was a trend toward greater stiffness in the 1.5 g group (p=0.139 vs. controls in the radius), and no change in strength. In the lumbar spine, no differences were seen due to vibration. Both vibration groups significantly reduced pyridinoline crosslinks, a collagen breakdown marker. They also significantly increased dynamic mineralization, MS/BS. Furthermore, osteoclasts were most numerous in the 1.5 g group (p≤ 0.05). These findings suggest that some benefits of WBV found in previous studies of young and mature rodent models may extend to an aging population. Density parameters indicated 0.5 g was more effective than 1.5 g. Serological markers, by contrast, favored 1.5 g, while biomechanically and histologically the results were mixed. Although the purported anabolic effect of WBV on bone homeostasis may depend on location and the parameter of interest, this emerging therapy at a minimum does not appear to compromise bone health by the measures studied here.
最近的研究表明，全身振动（WBV）可以改善某些临床情况和年龄段的骨骼健康状况。在老年人中，人们特别关注评估身体干预措施（如 WBV）改善协调、力量和运动速度的能力，这些能力有助于预防跌倒和骨折，保持独立生活的活动能力。目前的研究评估了 WBV 在衰老小鼠模型中的疗效。两种振动水平（0.5 和 1.5g）以 32Hz 的频率施加于 CB57BL/6 雄性小鼠（每组 9 只），从 18 个月大开始，持续 12 周，每天 30 分钟，使用新型旋转振动装置。以前的报告表明，这些小鼠的骨参数在 18 个月时开始大幅下降，相当于人类的 50 多岁。对股骨、桡骨和腰椎进行微计算机断层扫描（micro-CT）和生物力学评估，以确定这些 WBV 幅度和持续时间在衰老模型中的作用。还收集血清进行骨形成和分解标志物分析。组织学测定矿化表面和细胞计数。股骨四个区域的骨体积没有明显变化，但骨密度谱（BDS）中密度的均值呈上升趋势，两种振动水平产生相似的结果。该新参数代表常规密度直方图的积分。头部、颈部和骨干的高密度骨量统计学上有所增加。生物力学上，1.5g 组的刚度呈上升趋势（桡骨与对照组相比 p=0.139），强度无变化。在腰椎，由于振动没有差异。两组振动均显著降低吡啶交联物，一种胶原蛋白分解标志物。它们还显著增加了动态矿化，MS/BS。此外，破骨细胞在 1.5g 组中数量最多（p≤0.05）。这些发现表明，以前在年轻和成熟啮齿动物模型中进行的 WBV 研究中的一些益处可能扩展到老年人群。密度参数表明 0.5g 比 1.5g 更有效。相比之下，血清标志物有利于 1.5g，而生物力学和组织学结果则混杂不一。尽管 WBV 对骨内稳态的假定合成作用可能取决于位置和感兴趣的参数，但就目前的研究来看，这种新兴疗法至少不会通过所研究的措施损害骨骼健康。
