Takuma Daisuke, Guangchen Sun, Yokota Junko, Hamada Atsuhide, Onogawa Masahide, Yoshioka Saburo, Kusunose Masahiko, Miyamura Mitsuhiko, Kyotani Syojiro, Nishioka Yutaka
Department of Biomedical Science, Kochi Medical Graduate School, Oko-cho, Nankoku, Kochi, Japan.
Biol Pharm Bull. 2008 Feb;31(2):250-4. doi: 10.1248/bpb.31.250.
In this study, we investigated the effects of an Eriobotrya japonica seed extract (ESE) on mucositis using a 5-fluorouracil (5-FU)-induced mucositis hamster model. This model was prepared by intraperitoneally administering 90 mg/kg of 5-FU to hamsters on Day 1, scratching 1 cm(2) of the left cheek pouch of hamsters with a wire brush on Days 2, 3, and 4, and intraperitoneally administering 60 mg/kg of 5-FU on Day 5. Mucositis was evaluated based on the mucositis score at the mucositis site, left cheek pouch thickness, histological findings on HE staining, and plasma lipid peroxide levels. On Day 10, the mucositis score and left cheek pouch thickness in the ESE group were significantly lower than those in the tap water group. Histologically, the two groups showed a defect of the cheek pouch epithelium on Day 6. On Day 10, epithelial injury and bacterial infection were noted in the tap water group. However, in the ESE group, similar findings were not observed. On Day 6, the plasma lipid peroxide level in the tap water group was significantly higher than that in the normal group. In the ESE group, the plasma lipid peroxide level was significantly lower than that in the tap water group. These results suggest that ESE is useful for treating chemotherapy-induced mucositis.
在本研究中，我们使用5-氟尿嘧啶（5-FU）诱导的黏膜炎仓鼠模型，研究了枇杷种子提取物（ESE）对黏膜炎的影响。该模型的制备方法为：在第1天给仓鼠腹腔注射90 mg/kg的5-FU，在第2、3和4天用钢丝刷刮擦仓鼠左颊囊1 cm²的面积，在第5天腹腔注射60 mg/kg的5-FU。根据黏膜炎部位的黏膜炎评分、左颊囊厚度、苏木精-伊红（HE）染色的组织学结果以及血浆脂质过氧化物水平来评估黏膜炎。在第10天，ESE组的黏膜炎评分和左颊囊厚度显著低于自来水组。组织学上，两组在第6天均显示颊囊上皮缺损。在第10天，自来水组出现上皮损伤和细菌感染。然而，ESE组未观察到类似情况。在第6天，自来水组的血浆脂质过氧化物水平显著高于正常组。在ESE组，血浆脂质过氧化物水平显著低于自来水组。这些结果表明，ESE对治疗化疗引起的黏膜炎有效。
