Baylor College of Medicine, Houston, TX, USA.
J Neural Eng. 2010 Aug;7(4):045002. doi: 10.1088/1741-2560/7/4/045002. Epub 2010 Jul 19.
To study the complex synaptic interactions underpinning dendritic information processing in single neurons, experimenters require methods to mimic presynaptic neurotransmitter release at multiple sites with no physiological damage. We show that laser scanning systems built around large-aperture acousto-optic deflectors and high numerical aperture objective lenses provide the sub-millisecond, sub-micron precision necessary to achieve physiological, exogenous synaptic stimulation. Our laser scanning systems can produce the sophisticated spatio-temporal patterns of synaptic input that are necessary to investigate single-neuron dendritic physiology.
为了研究单个神经元中树突信息处理所必需的复杂突触相互作用，实验人员需要开发一种方法，在不对神经元造成任何生理损伤的情况下，在多个部位模拟突触前神经递质的释放。我们表明，基于大孔径声光偏转器和高数值孔径物镜的激光扫描系统，提供了实现生理、外源性突触刺激所需的亚毫秒、亚微米精度。我们的激光扫描系统可以产生复杂的突触输入时空模式，这对于研究单个神经元树突生理学是必要的。
