为中国东海一种具有重要经济价值的贻贝——波纹巴非蛤（贻贝科）开发微卫星标记。

Development of microsatellite markers for Mytilus coruscus (Mytilidae), an economically important mussel in the East China Sea.

作者信息

Guo B-Y, Wu C-W, Liu C-L, Zhu A-Y, Ye Y-Y

机构信息

Zhejiang Ocean University, National Engineering Research Center of Marine Facilities Aquaculture, Zhoushan, China.

出版信息

Genet Mol Res. 2013 Jul 30;12(3):2670-3. doi: 10.4238/2013.July.30.5.

DOI:10.4238/2013.July.30.5

PMID:23979892

Abstract

Twelve new polymorphic microsatellite loci were developed for the hard-shelled mussel, Mytilus coruscus. In 32 individuals from a wild population of coastal Zhoushan, Zhejiang Province, China, the number of alleles at these loci varied from 3 to 15, with a mean of 5.667. The mean observed and expected heterozygosities were 0.6927 and 0.6591, respectively. Among these polymorphic microsatellite loci, three (MC42, MC129, and MC180) significantly deviated from Hardy-Weinberg equilibrium after sequential Bonferroni's correction. All other microsatellite loci were in linkage equilibrium. These microsatellite loci will be useful for detecting genetic differences and for planning aquaculture management of M. coruscus.

摘要

为皱纹盘鲍开发了12个新的多态性微卫星位点。在中国浙江省舟山沿海的一个野生群体的32个个体中，这些位点的等位基因数量从3个到15个不等，平均为5.667个。观察到的平均杂合度和预期杂合度分别为0.6927和0.6591。在这些多态性微卫星位点中，经过连续Bonferroni校正后，有三个（MC42、MC129和MC180）显著偏离哈迪-温伯格平衡。所有其他微卫星位点处于连锁平衡。这些微卫星位点将有助于检测皱纹盘鲍的遗传差异，并用于规划其水产养殖管理。