鲻鱼（Mugil cephalus）多态性微卫星标记的开发与特性分析
Development and characterization of polymorphic microsatellite markers in the gray mullet (Mugil cephalus).
作者信息
Xu T-J, Sun D-Q, Shi G, Wang R-X
机构信息
Key Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, PR China.
出版信息
Genet Mol Res. 2010 Sep 8;9(3):1791-5. doi: 10.4238/vol9-3gmr909.
Using an (AG)13 enriched genomic library of Mugil cephalus, 12 polymorphic microsatellite loci were isolated and characterized in a test population; the number of alleles ranged from 2 to 11. The observed and expected heterozygosities ranged from 0.2593 to 0.8966 and from 0.3047 to 0.8454, respectively. Two loci deviated from Hardy-Weinberg equilibrium; linkage disequilibrium among the 12 loci was non-significant. These polymorphic microsatellite loci will be useful for genetic diversity analysis and molecule-assisted breeding of the gray mullet.
利用一个富含（AG）13的鲻鱼基因组文库，在一个测试群体中分离并鉴定了12个多态性微卫星位点；等位基因数量从2到11不等。观察到的杂合度和预期杂合度分别在0.2593至0.8966和0.3047至0.8454之间。两个位点偏离了哈迪-温伯格平衡；12个位点之间的连锁不平衡不显著。这些多态性微卫星位点将有助于鲻鱼的遗传多样性分析和分子辅助育种。
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