Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
Med Princ Pract. 2010;19(4):281-6. doi: 10.1159/000312714. Epub 2010 May 26.
The aims of this study were to determine the most effective solvent extract of mangosteen, anti-acne- inducing bacterial activity and the amount of alpha-mangostin, a major active component in each mangosteen fruit rind extract, using high-performance liquid chromatography (HPLC).
The fruit rinds of mangosteen were extracted with hexane, dichloromethane, ethanol and water. The extracts were tested for antibacterial activity against bacteria that induce acne, including Propionibacterium acnes and Staphylococcus epidermidis. Thin-layer chromatographic autobiography against these bacteria was also performed for each extract, while the alpha-mangostin content was analyzed using a validated HPLC method.
The dichloromethane extract exhibited the strongest antibacterial effect with minimum inhibitory concentration values for both bacterial species at 3.91 microg/ml, while the minimum bactericidal concentration values against P. acnes and S. epidermidis were 3.91 and 15.63 microg/ml, respectively. Thin-layer chromatographic autobiography indicated that alpha-mangostin was present in all extracts, except the water extract, and is a major active component against both P. acnes and S. epidermidis. Using HPLC, the dichloromethane extract yielded the highest content (46.21% w/w) of alpha-mangostin followed by the ethanol extract (18.03% w/w), the hexane extract (17.21% w/w) and the water extract (0.54% w/w).
Dichloromethane extract exhibited the strongest anti-acne-inducing bacterial effect and this extract yielded the highest amount of alpha-mangostin.
本研究旨在通过高效液相色谱法（HPLC）确定最有效的藤黄果提取物溶剂、抗粉刺诱导细菌活性以及每种藤黄果皮提取物中主要活性成分α-倒捻子素的含量。
采用正己烷、二氯甲烷、乙醇和水对藤黄果皮进行提取。测试提取物对痤疮诱导细菌（包括痤疮丙酸杆菌和表皮葡萄球菌）的抗菌活性。还对每种提取物进行了针对这些细菌的薄层色谱自传，同时使用经过验证的 HPLC 方法分析α-倒捻子素含量。
二氯甲烷提取物对两种细菌的最小抑菌浓度值均为 3.91μg/ml，表现出最强的抗菌作用，最小杀菌浓度值分别为 3.91μg/ml 和 15.63μg/ml。薄层色谱自传表明，除水提取物外，所有提取物均含有α-倒捻子素，是抗痤疮丙酸杆菌和表皮葡萄球菌的主要活性成分。通过 HPLC，二氯甲烷提取物的α-倒捻子素含量最高（46.21%w/w），其次是乙醇提取物（18.03%w/w）、正己烷提取物（17.21%w/w）和水提取物（0.54%w/w）。
二氯甲烷提取物表现出最强的抗粉刺诱导细菌作用，且该提取物中α-倒捻子素含量最高。
