Department of Bio-Nano Science and Engineering, Key Laboratory for Thin Film and Microfabrication Technology of Ministry of Education, National Key Laboratory of Micro/Nano Fabrication Technology,Research Institute of Micro/Nano Science and Technology, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China.
Nanoscale Res Lett. 2009 Sep 3;4(12):1469-74. doi: 10.1007/s11671-009-9422-7.
Quantum dot is a special kind of nanomaterial composed of periodic groups of II-VI, III-V or IV-VI materials. Their high quantum yield, broad absorption with narrow photoluminescence spectra and high resistance to photobleaching, make them become a promising labeling substance in biological analysis. Here, we report a quick and parallel analytical method based on quantum dots for ToRCH-related antibodies including Toxoplasma gondii, Rubella virus, Cytomegalovirus and Herpes simplex virus type 1 (HSV1) and 2 (HSV2). Firstly, we fabricated the microarrays with the five kinds of ToRCH-related antigens and used CdTe quantum dots to label secondary antibody and then analyzed 100 specimens of randomly selected clinical sera from obstetric outpatients. The currently prevalent enzyme-linked immunosorbent assay (ELISA) kits were considered as "golden standard" for comparison. The results show that the quantum dots labeling-based ToRCH microarrays have comparable sensitivity and specificity with ELISA. Besides, the microarrays hold distinct advantages over ELISA test format in detection time, cost, operation and signal stability. Validated by the clinical assay, our quantum dots-based ToRCH microarrays have great potential in the detection of ToRCH-related pathogens.
量子点是由 II-VI、III-V 或 IV-VI 材料周期性组合而成的特殊纳米材料。其具有高量子产率、宽吸收窄荧光光谱和高抗光漂白性，成为生物分析中一种有前途的标记物质。在这里，我们报告了一种基于量子点的快速并行分析方法，用于检测弓形虫、风疹病毒、巨细胞病毒和单纯疱疹病毒 1 型（HSV1）和 2 型（HSV2）等与 ToRCH 相关的抗体。首先，我们用五种与 ToRCH 相关的抗原制备了微阵列，并使用 CdTe 量子点标记了二级抗体，然后分析了 100 份随机选择的妇产科门诊患者的临床血清样本。目前流行的酶联免疫吸附测定（ELISA）试剂盒被认为是比较的“金标准”。结果表明，基于量子点标记的 ToRCH 微阵列与 ELISA 具有可比的灵敏度和特异性。此外，与 ELISA 检测模式相比，微阵列在检测时间、成本、操作和信号稳定性方面具有明显优势。通过临床检测验证，我们基于量子点的 ToRCH 微阵列在检测与 ToRCH 相关的病原体方面具有很大的潜力。
