Servicio de Análisis Clínicos, Sección de Bioquímica, Hospital Universitario Virgen de la Arrixaca, Murcia, Spain.
J Physiol Biochem. 2010 Sep;66(3):221-7. doi: 10.1007/s13105-010-0028-4. Epub 2010 Jul 22.
The biological effects of oxidized LDL (oxLDL) may contribute to initiation and progression of the atherosclerotic process, and the association between cardiovascular disease and oxidation of LDL has been largely demonstrated. The objectives of this study were to establish the reference values of oxidative stress biomarkers in a young healthy Spanish population to determine the concentration of oxLDL and its relationship with lipid profile and with these biomarkers. oxLDL, F(2)-isoprostanes, protein carbonyls (PC), and 8-hydroxy-2'-deoxyguanosine (8-OHdG) were determinate by ELISA in 72 healthy subjects. Antioxidant enzymes, superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione reductase (GR) were carried out on a Hitachi 912 analyzer; lipid profile were assayed using automated systems (Cobas 711, Roche Diagnostics). All statistics were analyzed by using SPSS for Windows 15.0. SPSS Inc, Chicago, IL, USA. (Normal mean reference values): oxLDL (63.23 +/- 16.23 U/L), (Male/Female 68.06 +/- 17.69/58.39 +/- 13.6 U/L), F(2)-isoprostanes (2.26 +/- 0.9 microg/g creatinine), PC (0.34 +/- 0.15 nmol/mg), 8-OHdG (23.27 +/- 10.58 ng/ml), SOD (931.97 +/- 271.09 U/g Hb), GR (46.56 +/- 11.68 U/L), GPx (27.58 +/- 6.89 U/gHb (Male/Female 25.91 +/- 5.03/29.2 +/- 8.07 U/L)). OxLDL (63.23 U/L) was significantly (p < 0.05) positively correlated with BMI (22.53 Kg/m(2)), total cholesterol (175.79 mg/dl), triglycerides (87.58 mg/dl), LDL cholesterol (96.25 mg/dl), and uric acid (4.78 mg/dl), while negatively correlated with HDL-cholesterol (62.25 mg/dl). We have found different correlation between oxLDL and isoprostanes by gender with the rest of parameters. Normal reference values have been found significantly different for oxLDL and GPx by gender. Oxidized LDL is correlated with lipid profile but not with the oxidative stress biomarkers. Urinary isoprostanes are positively correlated with triglycerides and negatively with GR and GPx.
氧化型低密度脂蛋白（oxLDL）的生物学效应可能导致动脉粥样硬化过程的启动和进展，并且心血管疾病与 LDL 氧化之间的关联已得到广泛证明。本研究的目的是确定年轻健康的西班牙人群中氧化应激生物标志物的参考值，以确定 oxLDL 的浓度及其与脂质谱和这些生物标志物的关系。通过 ELISA 在 72 名健康受试者中测定 oxLDL、F(2)-异前列腺素、蛋白羰基（PC）和 8-羟基-2'-脱氧鸟苷（8-OHdG）。超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）和谷胱甘肽还原酶（GR）的抗氧化酶在日立 912 分析仪上进行测定；脂质谱使用自动化系统（Cobas 711，罗氏诊断）进行测定。所有统计数据均使用 Windows 15.0 SPSS 进行分析。SPSS Inc，芝加哥，IL，美国。（正常均值参考值）：oxLDL（63.23±16.23 U/L），（男/女 68.06±17.69/58.39±13.6 U/L），F(2)-异前列腺素（2.26±0.9μg/g 肌酐），PC（0.34±0.15 nmol/mg），8-OHdG（23.27±10.58ng/ml），SOD（931.97±271.09 U/gHb），GR（46.56±11.68 U/L），GPx（27.58±6.89 U/gHb（男/女 25.91±5.03/29.2±8.07 U/L））。oxLDL（63.23 U/L）与 BMI（22.53 Kg/m2）、总胆固醇（175.79 mg/dl）、甘油三酯（87.58 mg/dl）、LDL 胆固醇（96.25 mg/dl）和尿酸（4.78 mg/dl）呈显著正相关（p<0.05），与高密度脂蛋白胆固醇（62.25 mg/dl）呈负相关。我们发现 oxLDL 和异前列腺烷之间存在不同的性别相关性与其余参数。oxLDL 和 GPx 的性别参考值差异显著。氧化型低密度脂蛋白与脂质谱相关，但与氧化应激生物标志物无关。尿异前列腺素与甘油三酯呈正相关，与 GR 和 GPx 呈负相关。
