Department of Molecular Genetics & Microbiology and Center for Virology, Duke University Medical Center, Durham, NC 27710, USA.
Virology. 2010 Sep 30;405(2):592-9. doi: 10.1016/j.virol.2010.06.036. Epub 2010 Jul 24.
Rhesus rhadinovirus (RRV), a primate gamma-herpesvirus related to human Kaposi's sarcoma-associated herpesvirus (KSHV), causes a similar pattern of pathogenesis. Previously, RRV was shown to express 7 pre-microRNAs (pre-miRNAs) in latently infected cells. Using deep sequencing, we analyzed the pattern of small RNA expression in vivo using latently RRV-infected B-cell lymphoma and retroperitoneal fibromatosis tissues. We identified 15 virally encoded pre-miRNAs in both tumors, including all previously reported RRV pre-miRNAs. Although all 15 RRV pre-miRNAs, like all 12 KSHV pre-miRNAs, are located 3' to the conserved viral ORF71 gene and in the same transcriptional orientation, only one RRV miRNA is homologous to a KSHV miRNA. One previously identified RRV miRNA, miR-rR1-3, is actually a miRNA offset RNA (moRNA) derived from sequences located adjacent to pre-miR-rR1-3. Several other RRV-derived moRNAs were obtained, including one recovered >600 times. Together, this research provides a comprehensive list of the miRNAs and moRNAs encoded by RRV.
恒河猴疱疹病毒（RRV）是一种与人类卡波西肉瘤相关疱疹病毒（KSHV）相关的灵长类动物γ疱疹病毒，可引起相似的发病模式。此前，RRV 已被证明在潜伏感染的细胞中表达 7 个前 microRNA（pre-miRNA）。我们使用深度测序，分析了潜伏 RRV 感染的 B 细胞淋巴瘤和腹膜后纤维瘤组织中体内小 RNA 表达的模式。我们在这两种肿瘤中都鉴定出了 15 种病毒编码的前 microRNA，包括所有先前报道的 RRV 前 microRNA。尽管所有 15 种 RRV 前 microRNA，与所有 12 种 KSHV 前 microRNA 一样，都位于保守的病毒 ORF71 基因的 3'端，并处于相同的转录方向，但只有一种 RRV microRNA 与 KSHV microRNA 同源。之前鉴定的一种 RRV microRNA，miR-rR1-3，实际上是一种源自 pre-miR-rR1-3 附近序列的 microRNA 偏移 RNA（moRNA）。获得了其他几种 RRV 衍生的 moRNA，包括一种被回收了超过 600 次的 moRNA。总之，这项研究提供了 RRV 编码的 microRNA 和 moRNA 的综合清单。
