对整个活体脊椎动物的膜脂有序性进行成像。
Imaging membrane lipid order in whole, living vertebrate organisms.
机构信息
Center for Vascular Research, University of New South Wales, Sydney, Australia.
出版信息
Biophys J. 2010 Jul 7;99(1):L7-9. doi: 10.1016/j.bpj.2010.04.022.
Abstract
We report the first imaging of membrane lipid order in a whole, living vertebrate organism. This was achieved with the phase-sensitive, membrane-partitioning probe Laurdan in conjunction with multiphoton microscopy to image cell membranes in various tissues of live zebrafish embryos in three dimensions, including hindbrain, retina, muscle, gut, and kidney. The data also allowed quantitative analysis of membrane order, which showed high lipid order in the apical surfaces of polarized epithelial cells. The transition of membrane order imaging from cultured cell lines to living organisms is an important step forward in understanding the physiological relevance of membrane microdomains including lipid rafts.
摘要
我们报告了在整个活体脊椎动物中对膜脂有序性的首次成像。这是通过具有相位敏感性的、与膜结合的荧光探针 Laurdan 与多光子显微镜相结合来实现的，以便在活体斑马鱼胚胎的各种组织中对细胞膜进行三维成像，包括后脑、视网膜、肌肉、肠道和肾脏。这些数据还允许对膜有序性进行定量分析，结果表明在极化上皮细胞的顶表面处具有较高的脂质有序性。将膜有序性成像从培养的细胞系推进到活体生物，是理解包括脂筏在内的膜微区的生理相关性的重要一步。
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