Broderson S H, Patton D L, Stahl W L
J Cell Biol. 1978 May;77(2):R13-7. doi: 10.1083/jcb.77.2.r13.
A histochemical technique for the demonstration of K+-rho-nitrophenylphosphatase (K+-rhoNPPase) activity, a component of the Na+,K+-ATPase, has been applied at the fine structural level in the somatosensory cortex of the rat. Reaction product was consistenly found in dendrites and in association with the cytoplasmic aspect of the dendritic plasmalemma. Reaction product often filled portions of the tubular smooth endoplasmic reticulum in these processes. The results of these studies are interpreted to indicate that enzymatic activity is associated with large-and small-diameter dendrites. No convincing evidence of high activity was found in glial profiles. The importance of neurons and their dendrites in active transport of sodium and potassium ions in the cerebral cortex may be more significant than indicated by studies with isolated neurons and glia.
一种用于显示K⁺-ρ-硝基苯磷酸酶（K⁺-ρNPPase）活性（Na⁺,K⁺-ATP酶的一个组成部分）的组织化学技术已应用于大鼠体感皮层的精细结构水平研究。反应产物始终存在于树突中，并与树突质膜的胞质面相关。在这些过程中，反应产物常常充满管状滑面内质网的部分区域。这些研究结果被解释为表明酶活性与大、小直径的树突相关。在神经胶质细胞形态中未发现高活性的令人信服的证据。神经元及其树突在大脑皮层钠钾离子主动转运中的重要性可能比用分离的神经元和神经胶质细胞进行的研究所显示的更为显著。
