Institute of Chinese Medical Sciences, University of Macau, Macao, China.
Molecules. 2010 Jun 11;15(6):4227-41. doi: 10.3390/molecules15064227.
Free and polymeric carbohydrates in Cordyceps, a valued edible mushroom and well-known traditional Chinese medicine, were determined using stepwise pressurized liquid extraction (PLE) extraction and GC-MS. Based on the optimized PLE conditions, acid hydrolysis and derivatization, ten monosaccharides, namely rhamnose, ribose, arabinose, xylose, mannose, glucose, galactose, mannitol, fructose and sorbose in 13 samples of natural and cultured Cordyceps were qualitatively and quantitatively analyzed and compared with myo-inositol hexaacetate as internal standard. The results showed that natural C. sinensis contained more than 7.99% free mannitol and a small amount of glucose, while its polysaccharides were usually composed of mannose, glucose and galactose with a molar ratio of 1.00:16.61-3.82:1.60-1.28. However, mannitol in cultured C. sinensis and cultured C. militaris were less than 5.83%, and free glucose was only detected in a few samples, while their polysaccharides were mainly composed of mannose, glucose and galactose with molar ratios of 1.00:3.01-1.09:3.30-1.05 and 1.00:2.86-1.28:1.07-0.78, respectively. Natural and cultured Cordyceps could be discriminated by hierarchical clustering analysis based on its free carbohydrate contents.
采用分步加压液体萃取（PLE）提取和 GC-MS 法测定了蛹虫草中游离和聚合碳水化合物。基于优化的 PLE 条件、酸水解和衍生化，对 13 个天然和培养蛹虫草样品中的 10 种单糖，即鼠李糖、核糖、阿拉伯糖、木糖、甘露糖、葡萄糖、半乳糖、甘露醇、果糖和山梨糖，与肌醇六乙酸酯作为内标进行了定性和定量分析，并与肌醇六乙酸酯进行了比较。结果表明，天然蛹虫草含有超过 7.99%的游离甘露醇和少量的葡萄糖，而其多糖通常由甘露糖、葡萄糖和半乳糖组成，摩尔比为 1.00:16.61-3.82:1.60-1.28。然而，培养的蛹虫草和蛹虫草中的甘露醇含量均小于 5.83%，游离葡萄糖仅在少数样品中检测到，而它们的多糖主要由甘露糖、葡萄糖和半乳糖组成，摩尔比分别为 1.00:3.01-1.09:3.30-1.05 和 1.00:2.86-1.28:1.07-0.78。基于游离碳水化合物含量，可通过层次聚类分析对天然和培养蛹虫草进行区分。
