Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, Virginia, USA.
Nat Struct Mol Biol. 2010 Aug;17(8):1027-9. doi: 10.1038/nsmb.1856. Epub 2010 Jul 25.
MSL3 resides in the MSL (male-specific lethal) complex, which upregulates transcription by spreading the histone H4 Lys16 (H4K16) acetyl mark. We discovered a DNA-dependent interaction of MSL3 chromodomain with the H4K20 monomethyl mark. The structure of a ternary complex shows that the DNA minor groove accommodates the histone H4 tail, and monomethyllysine inserts in a four-residue aromatic cage in MSL3. H4K16 acetylation antagonizes MSL3 binding, suggesting that MSL function is regulated by a combination of post-translational modifications.
MSL3 位于雄性特异致死 (MSL) 复合物中，通过延伸组蛋白 H4 Lys16 (H4K16) 乙酰化标记来上调转录。我们发现 MSL3 色氨酸结构域与 H4K20 单甲基标记之间存在 DNA 依赖性相互作用。一个三元复合物的结构表明 DNA 小沟容纳组蛋白 H4 尾部，单甲基赖氨酸插入 MSL3 中的四残基芳香笼中。H4K16 乙酰化拮抗 MSL3 结合，表明 MSL 功能受翻译后修饰的组合调控。
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