Department of Biotechnology, Alagappa University, Karaikudi, 630 003, Tamil Nadu, India.
Appl Microbiol Biotechnol. 2010 Sep;88(1):341-58. doi: 10.1007/s00253-010-2777-y. Epub 2010 Jul 28.
According to the Centers for Disease Control and Prevention, biofilms cause 65% of infections in developed countries. Pseudomonas aeruginosa biofilm cause life threatening infections in cystic fibrosis infection and they are 1,000 times more tolerant to antibiotic than the planktonic cells. As quorum sensing, hydrophobicity index and extracellular polysaccharide play a crucial role in biofilm formation, extracts from 46 marine bacterial isolates were screened against these factors in P. aeruginosa. Eleven extracts showed antibiofilm activity. Extracts of S6-01 (Bacillus indicus = MTCC 5559) and S6-15 (Bacillus pumilus = MTCC 5560) inhibited the formation of PAO1 biofilm up to 95% in their Biofilm Inhibitory Concentration(BIC) of 50 and 60 microg/ml and 85% and 64% in the subinhibitory concentrations (1/4 and 1/8 of the BIC, respectively). Furthermore, the mature biofilm was disrupted to 70-74% in their BIC. The antibiofilm compound from S6-15 was partially purified using solvent extraction followed by TLC and silica column and further characterized by IR analysis. Current study for the first time reveals the antibiofilm and antiquorum-sensing activity of B. pumilus, B. indicus, Bacillus arsenicus, Halobacillus trueperi, Ferrimonas balearica, and Marinobacter hydrocarbonoclasticus from marine habitat.
根据疾病控制和预防中心的数据，生物膜导致了发达国家 65%的感染。铜绿假单胞菌生物膜会导致囊性纤维化感染的生命威胁性感染，它们对抗生素的耐受度比浮游细胞高出 1000 倍。由于群体感应、疏水性指数和细胞外多糖在生物膜形成中起着关键作用，因此从 46 株海洋细菌分离物中筛选出了针对这些因素的提取物。有 11 种提取物具有抗生物膜活性。S6-01（印度芽孢杆菌=MTCC 5559）和 S6-15（短小芽孢杆菌=MTCC 5560）的提取物在其生物膜抑制浓度（BIC）为 50 和 60 μg/ml 时，可抑制 PAO1 生物膜的形成，达到 95%，在亚抑菌浓度（BIC 的 1/4 和 1/8，分别）下，抑制率为 85%和 64%。此外，成熟生物膜在其 BIC 中被破坏了 70-74%。从 S6-15 中分离出的抗生物膜化合物通过溶剂萃取、TLC 和硅胶柱进行部分纯化，并通过 IR 分析进一步表征。本研究首次揭示了海洋生境中的短小芽孢杆菌、印度芽孢杆菌、砷酸杆菌、盐单胞菌、费氏弧菌和烃降解海杆菌的抗生物膜和抗群体感应活性。
