Department of Ophthalmology, Nanshan Hospital of Guangdong Medical College, Shenzhen, PR China.
Curr Eye Res. 2010 Aug;35(8):685-97. doi: 10.3109/02713681003793136.
To study the relationship between corneal lymphangiogenesis and inflammation in alkali burned corneas.
Rat corneal lymphatic and blood vessels were labeled and distinguished by whole mount immunofluorescence and 5'-nase-alkaline phosphatase (5'-NA-ALP) double enzyme-histochemistry. Then, lymphatic vessel areas (LVA) and lymphatic vessel counting (LVC) were examined. Corneal inflammation was evaluated by inflammation index (IF) grading, histopathology, electron microscope, and polymorphonuclear leukocyte (PMN) infiltration. The relationship between LVC, LVA, IF, and PMN was examined, respectively. In addition, corneal lymphatic vessels of eleven human alkali burned corneas were examined by lymphatic vessel endothelial receptor (LYVE-1) immunohistochemistry.
Corneal lymphangiogenesis occurred on Day 3, reached the peak at the end of two weeks, and disappeared five weeks after alkaline burns. Both LVA and LVC were strongly and positively correlated with IF after corneal alkaline burns. However, the relationship between LVC and PMN, between LVA and PMN were significant but converse. Among eleven human alkali burned corneas, corneal lymphangiogenesis was present in three corneas.
Corneal lymphagiogenesis develops after alkaline burns and correlates closely with corneal inflammation.
研究碱性烧伤角膜中角膜淋巴管生成与炎症的关系。
通过全层免疫荧光和 5'-核苷酸酶-碱性磷酸酶（5'-NA-ALP）双重酶组织化学，对大鼠角膜淋巴管和血管进行标记和区分。然后，检查淋巴管面积（LVA）和淋巴管计数（LVC）。通过炎症指数（IF）分级、组织病理学、电子显微镜和多形核白细胞（PMN）浸润来评估角膜炎症。分别检查 LVC、LVA、IF 和 PMN 之间的关系。此外，通过淋巴管内皮受体（LYVE-1）免疫组织化学检查了 11 例人碱烧伤角膜的淋巴管。
角膜碱性烧伤后第 3 天发生淋巴管生成，两周末达到高峰，碱性烧伤后 5 周消失。碱性烧伤后，LVA 和 LVC 与 IF 均呈强烈正相关。然而，LVC 与 PMN 之间、LVA 与 PMN 之间的关系虽然显著，但却是相反的。在 11 例人碱烧伤角膜中，有 3 例角膜淋巴管生成。
碱性烧伤后会发生角膜淋巴管生成，与角膜炎症密切相关。
