Departamento de Análises Clínicas e Departamento de Ciências Biológicas, UNESP, R. Expedicionariosdo Brasil 1621, 14801-902 Araraquara, SP, Brazil.
Pharm Biol. 2010 Aug;48(8):878-82. doi: 10.3109/13880200903303471.
Mycobacterium tuberculosis is responsible for over 8 million cases of tuberculosis (TB) annually. Natural products may play important roles in the chemotherapy of TB. The antimycobacterial activity and the innate immune response of methanol (METH) and dichloromethane (DCM) extracts of Indigofera suffruticosa Miller (Fabaceae) were evaluated. We observed that the minimum inhibitory concentrations (MICs) for METH and DCM extracts were 125 and 1000 microg/mL, respectively. However, they were able to induce the innate immune response through the production of high levels of NO and TNF-alpha (p < 0.001) by peritoneal exudate cells (PECs). These results suggest that I. suffruticosa extracts may have an important immunological role in the control of TB once macrophage activity is induced by them.
结核分枝杆菌每年导致超过 800 万例结核病（TB）。天然产物可能在结核病的化学治疗中发挥重要作用。评价了木蓝（豆科）甲醇（METH）和二氯甲烷（DCM）提取物的抗分枝杆菌活性和固有免疫反应。我们观察到 METH 和 DCM 提取物的最低抑菌浓度（MIC）分别为 125 和 1000μg/mL。然而，它们能够通过腹腔渗出细胞（PECs）产生高水平的 NO 和 TNF-α（p < 0.001）来诱导固有免疫反应。这些结果表明，一旦巨噬细胞被木蓝提取物激活，它可能在控制结核病方面发挥重要的免疫作用。
