Clínica Corachan, Centro de Infertilidad y Reproducción Humana (CIRH), Barcelona, Spain.
Fertil Steril. 2011 Jan;95(1):277-80. doi: 10.1016/j.fertnstert.2010.06.071. Epub 2010 Aug 2.
To compare the quality of sperm samples obtained after density-gradient centrifugation and swim-up by performing a nuclear structural analysis with high magnification microscopy at ×8,000.
Prospective and randomized split-sample study.
Reproductive Medicine Center.
PATIENT(S): Sperm samples from 53 male-partners of couples undergoing infertility treatment.
INTERVENTION(S): Samples were analyzed by high magnification microscopy before and after preparation and classified according the level of nuclear vacuolization.
MAIN OUTCOME MEASURE(S): Recovery rate, motility rate and percentage of sperm scoring each of 4 grades of vacuolization in fresh and processed sample.
RESULT(S): Both sperm processing methods, swim-up and density gradient centrifugation, allow the selection of sperm with lower nuclear vacuolization and presumably lower DNA fragmentation than the whole ejaculate.
CONCLUSION(S): Swim-up produces samples with less vacuolization, but the recovery rate is also lower. The choice of processing technique would then depend on whether intrauterine insemination, IVF or ICSI/IMSI is to be performed. A prospective randomized study scoring fertility rates would be necessary to directly access the influence of these methods on the fertility outcome.
通过在×8000 高倍显微镜下进行核结构分析，比较密度梯度离心法和游移法获取的精子样本质量。
前瞻性、随机分组研究。
生殖医学中心。
接受不孕治疗的夫妇中 53 名男性伴侣的精子样本。
在准备前后，使用高倍显微镜对样本进行分析，并根据核空泡化程度进行分类。
新鲜样本和处理样本中各级核空泡化精子的回收率、活力率和比例。
两种精子处理方法（游移法和密度梯度离心法）都可以选择核空泡化程度较低、DNA 碎片化程度较低的精子，而不是整个精液。
游移法产生的样本空泡化程度较低，但回收率也较低。那么，处理技术的选择将取决于是否要进行宫腔内人工授精、体外受精或卵胞浆内单精子注射/形态选择。有必要进行一项前瞻性随机研究，对生育力评分，以直接评估这些方法对生育结果的影响。
