意大利拉斯佩齐亚环境空气颗粒物中15种多环芳烃（PAH）与总PAH组分致突变性之间的相关性。

Correlations between 15 polycyclic aromatic hydrocarbons (PAH) and the mutagenicity of the total PAH fraction in ambient air particles in La Spezia (Italy).

作者信息

Barale R, Giromini L, Ghelardini G, Scapoli C, Loprieno N, Pala M, Valerio F, Barrai I

机构信息

Istituto di Zoologia, Università di Ferrara, Italy.

出版信息

Mutat Res. 1991 Jul;249(1):227-41. doi: 10.1016/0027-5107(91)90150-m.

DOI:10.1016/0027-5107(91)90150-m

PMID:2067536

Abstract

Airborne particulate matter has been monitored 4 times a month for 1 year (1988) in the city of La Spezia (Italy). The polycyclic aromatic hydrocarbon (PAH) fractions were extracted, purified and characterized for the content of 15 individual PAH. In general when concentrations of individual PAH were compared statistical correlation was obtained. Mutagenicity studies were performed by the use of the Ames plate test with the Salmonella strains TA98, TA100, TA98NR and TA98DNP6 with and without metabolic activation (S9 mix). The TA98 strain was by far the most responsive and the S9 mix was absolutely required as expected when PAH are assayed. Besides mutagenicity, toxicity was also considered and it proved to be correlated with mutagenicity in TA98, +S9. The TA98NR and TA98DNP6 strains showed no appreciable differences from the parental strain TA98 indicating the absence of significant amounts of direct-acting nitro derivatives in our PAH samples. Of the 15 PAH considered in this study the amounts of cyclopental[c,d]pyrene (CPP) correlated best with mutagenicity. The role of CPP in contributing to the indirect mutagenicity of urban air PAH samples is discussed.

摘要

1988年，在意大利拉斯佩齐亚市对空气中的颗粒物进行了为期一年的监测，每月监测4次。提取、纯化了多环芳烃（PAH）馏分，并对15种单个PAH的含量进行了表征。一般来说，比较单个PAH的浓度时会得到统计相关性。通过使用Ames平板试验，对沙门氏菌菌株TA98、TA100、TA98NR和TA98DNP6进行了致突变性研究，试验分别在有和没有代谢活化（S9混合物）的情况下进行。TA98菌株的反应最为明显，在检测PAH时，正如预期的那样，绝对需要S9混合物。除了致突变性外，还考虑了毒性，结果证明其与TA98、+S9中的致突变性相关。TA98NR和TA98DNP6菌株与亲本菌株TA98相比没有明显差异，这表明我们的PAH样品中不存在大量直接作用的硝基衍生物。在本研究中考虑的15种PAH中，环戊并[c,d]芘（CPP）的含量与致突变性的相关性最好。讨论了CPP在城市空气PAH样品间接致突变性中的作用。