Department of Radiation Biology, Institute for Cancer Research, Oslo University Hospital, Montebello, 0310 Oslo, Norway.
Pharm Res. 2010 Oct;27(10):2213-20. doi: 10.1007/s11095-010-0227-2. Epub 2010 Jul 31.
To determine the impact of skin pretreatment with microneedles (MNs) on ALA- and MAL-induced protoporphyrin IX (PpIX) production, as well as MN impact on pain sensations during light exposure and erythema after PDT.
The skin of 14 healthy volunteers was preteated with MNs. Equal amounts of creams containing 2%, 8% and 16% (w/w) ALA and MAL were applied on 1 cm(2) areas for 4 h. Additionally, 16% ALA and MAL creams were applied for 24 h. Afterwards, PpIX fluorescence spectra were measured. Sixteen percent ALA and MAL spots were exposed to red light (632 nm, 77 mW/cm(2)). Time for pain to occur was measured in seconds, and erythemal response was monitored up to 6 h after the end of the light exposure.
Use of MNs increased the PpIX fluorescence after 4 h incubation time with 2% and 8% ALA or MAL, but not with 16% ALA or MAL. Pretreatment with MNs did not increase the pain sensations during light exposure, nor did it influence erythema occurrence.
MNs are a promising tool for improving the efficiency of topical PDT by improving the cutaneous delivery of ALA and MAL, without increase in side effects.
确定微针（MN）预处理对 ALA 和 MAL 诱导的原卟啉 IX（PpIX）产生的影响，以及 MN 对光暴露期间疼痛感觉和 PDT 后红斑的影响。
将 MN 预处理于 14 名健康志愿者的皮肤。将含有 2%、8%和 16%（w/w）ALA 和 MAL 的乳膏等份应用于 1cm²区域，持续 4 小时。此外，将 16%的 ALA 和 MAL 乳膏应用 24 小时。之后，测量 PpIX 荧光光谱。将 16%的 ALA 和 MAL 斑点暴露于红光（632nm，77mW/cm²）。以秒为单位测量疼痛发生的时间，并在光暴露结束后 6 小时内监测红斑反应。
使用 MNs 可增加 4 小时孵育时间内 2%和 8%ALA 或 MAL 的 PpIX 荧光，但 16%ALA 或 MAL 则不然。MNs 预处理不会增加光暴露期间的疼痛感觉，也不会影响红斑的发生。
MNs 是一种有前途的工具，通过改善 ALA 和 MAL 的皮肤递送，提高局部 PDT 的效率，而不会增加副作用。
