Instituto de Ganadería de Montaña, CSIC-ULE, Finca Marzanas. 24346 Grulleros, León, Spain.
Parasitol Res. 2010 Oct;107(5):1205-11. doi: 10.1007/s00436-010-1989-5. Epub 2010 Aug 3.
The aim of this study was to compare the efficacy of triclabendazole (TCBZ) and nitroxynil against a TCBZ-resistant Fasciola hepatica strain in a naturally infected sheep flock. The efficacies were measured by the faecal egg count reduction test. The level of F. hepatica antigens was tested in faeces; and haematological indices such as total proteins (TP), albumin, hepatic enzymes and total IgG were also studied. The results confirmed the resistance of F. hepatica against TCBZ in the flock with an efficacy during the first month post-treatment between 59.4% and 73.8%. In the nitroxynil group, the efficacy during the same period ranged between 81.3% and 86%, likely because the efficacy of this drug against 7- to 9-week-old immature stages is only 50-90%. Anemia was showed in all groups and white blood cells were always higher than the reference range. The values of TP and albumin were within normal range in most of the sheep, and an increase in hepatic enzymes confirmed the liver damage. Regarding total IgG, some negative correlations were found with egg excretion, and in relation to the level of antigens in faeces, these ones decreased immediately after treatment. We conclude that nitroxynil could be an alternative in case of TCBZ resistance.
本研究旨在比较三氯苯达唑（TCBZ）和硝氯酚对自然感染绵羊群体中 TCBZ 耐药的肝片吸虫的疗效。通过粪便卵计数减少试验来测量疗效。在粪便中检测肝片吸虫抗原水平；并研究了总蛋白（TP）、白蛋白、肝酶和总 IgG 等血液学指标。结果证实了羊群中肝片吸虫对 TCBZ 的耐药性，治疗后第一个月的疗效在 59.4%至 73.8%之间。在硝氯酚组中，同期疗效在 81.3%至 86%之间，这可能是因为该药对 7 至 9 周龄的未成熟阶段的疗效仅为 50-90%。所有组均出现贫血，白细胞始终高于参考范围。大多数绵羊的 TP 和白蛋白值均在正常范围内，肝酶的升高证实了肝损伤。关于总 IgG，与卵排泄存在一些负相关，并且与粪便中抗原的水平有关，这些在治疗后立即下降。我们得出结论，在 TCBZ 耐药的情况下，硝氯酚可能是一种替代药物。
