Department of Environmental Health and Institute of Health and Environmental, School of Public Health, Seoul National University, Seoul, Republic of Korea.
ISME J. 2011 Feb;5(2):362-5. doi: 10.1038/ismej.2010.120. Epub 2010 Aug 5.
Microbial compositions of human and animal feces from South Korea were analyzed and characterized. In total, 38 fecal samples (14 healthy adult humans, 6 chickens, 6 cows, 6 pigs and 6 geese) were analyzed by 454 pyrosequencing of the V2 region of the 16S rRNA gene. Four major phyla, Actinobacteria, Proteobacteria, Firmicutes and Bacteroidetes, were identified in the samples. Principal coordinate analysis suggested that microbiota from the same host species generally clustered, with the exception of those from humans, which exhibited sample-specific compositions. A network-based analysis revealed that several operational taxonomic units (OTUs), such as Lactobacillus sp., Clostridium sp. and Prevotella sp., were commonly identified in all fecal sources. Other OTUs were present only in fecal samples from a single organism. For example, Yania sp. and Bifidobacterium sp. were identified specifically in chicken and human fecal samples, respectively. These specific OTUs or their respective biological markers could be useful for identifying the sources of fecal contamination in water by microbial source tracking.
对来自韩国的人和动物粪便的微生物组成进行了分析和鉴定。总共分析了 38 个粪便样本（14 个健康成年人、6 只鸡、6 头牛、6 头猪和 6 只鹅），采用的方法是对 16S rRNA 基因 V2 区进行 454 焦磷酸测序。在样本中鉴定出了四个主要的门，放线菌门、变形菌门、厚壁菌门和拟杆菌门。主坐标分析表明，来自同一宿主物种的微生物群通常聚类，除了人类的样本，它们表现出特定的样本组成。基于网络的分析表明，一些操作分类单元（OTUs），如乳酸杆菌属、梭菌属和普雷沃氏菌属，在所有粪便来源中都被普遍鉴定出来。其他 OTUs 仅存在于单一生物体的粪便样本中。例如，亚尼娅菌属和双歧杆菌属分别特异性地存在于鸡和人粪便样本中。这些特定的 OTUs 或它们各自的生物标志物可用于通过微生物源追踪识别水中粪便污染的来源。
