Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Department of Pathology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
J Med Microbiol. 2010 Nov;59(Pt 11):1311-1316. doi: 10.1099/jmm.0.021139-0. Epub 2010 Aug 5.
In this study, PCR-RFLP analysis (PRA) targeting hsp65 and rpoB gene regions was evaluated for the identification of mycobacterial species isolated from Malaysian patients. Overall, the hsp65 PRA identified 92.2 % of 90 isolates compared to 85.6 % by the rpoB PRA. With 47 rapidly growing species, the hsp65 PRA identified fewer (89.4 %) species than the rpoB PRA (95.7 %), but with 23 slow-growing species the reverse was true (91.3 % identification by the hsp65 PRA but only 52.5 % by the rpoB PRA). There were 16 isolates with discordant PRA results, which were resolved by 16S rRNA and hsp65 gene sequence analysis. The findings in this study suggest that the hsp65 PRA is more useful than the rpoB PRA for the identification of Mycobacterium species, particularly with the slow-growing members of the genus. In addition, this study reports 5 and 12 novel restriction patterns for inclusion in the hsp65 and rpoB PRA algorithms, respectively.
在这项研究中，针对 hsp65 和 rpoB 基因区域的 PCR-RFLP 分析（PRA）用于鉴定从马来西亚患者中分离的分枝杆菌物种。总体而言，与 rpoB PRA 相比，hsp65 PRA 鉴定了 90 个分离株中的 92.2%。对于 47 种快速生长的物种，hsp65 PRA 鉴定的物种（89.4%）少于 rpoB PRA（95.7%），但对于 23 种缓慢生长的物种，情况则相反（hsp65 PRA 鉴定的物种为 91.3%，而 rpoB PRA 仅为 52.5%）。有 16 个分离株的 PRA 结果不一致，通过 16S rRNA 和 hsp65 基因序列分析解决了这些结果。本研究的结果表明，与 rpoB PRA 相比，hsp65 PRA 更有助于鉴定分枝杆菌物种，特别是对于该属的缓慢生长成员。此外，本研究报告了 5 种和 12 种新的限制图谱，分别纳入 hsp65 和 rpoB PRA 算法中。
